The enzyme catalyzes formation of the ureido ring of dethiobiotin (DTB) from (7R,8S)-7,8-diaminononanoic acid (DAPA) and carbon dioxide in the presence of ATP and divalent metal ions (e.g., Mg2+) (PMID9125485)
CATALYTIC METAL(S)
| Metal/Site Information | |||||
| Residue in MACiE* | Atom in MACiE* | Residue Type in MACiE | Physiological Metal |
Site Type |
Function(s) |
| MG 901 x | MG | magnesium | divalent cation | mononuclear | Electrostatic stabiliser Coordinates substrate Increases electrophilicity |
| MG 902 x | MG | magnesium | divalent cation | mononuclear | Electrostatic stabiliser Coordinates substrate Increases electrophilicity |
*It refers to the MACiE reference pdb: 1bs1
| Metal/s Properties in Resting State | ||||||
| MG 901 x | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | unknownGeometry | |||||
| Coordination Number | unknown | |||||
| Notes | - | |||||
| MG 902 x | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | unknownGeometry | |||||
| Coordination Number | unknown | |||||
| Notes | - | |||||
| References |
| -Käck H, Gibson KJ, Lindqvist Y, Schneider G Snapshot of a phosphorylated substrate intermediate by kinetic crystallography. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5495-500.(MEDLINE:9576910) |
| -Yang G, Sandalova T, Lohman K, Lindqvist Y, Rendina AR Active site mutants of Escherichia coli dethiobiotin synthetase: effects of mutations on enzyme catalytic and structural properties. Biochemistry. 1997 Apr 22;36(16):4751-60.(MEDLINE:9125495) |
| -Käck H, Sandmark J, Gibson KJ, Schneider G, Lindqvist Y Crystal structure of two quaternary complexes of dethiobiotin synthetase, enzyme-MgADP-AlF3-diaminopelargonic acid and enzyme-MgADP-dethiobiotin-phosphate; implications for catalysis. Protein Sci. 1998 Dec;7(12):2560-6.(MEDLINE:9865950) |