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Service Announcement

We will be retiring these pages on the 31st of January, 2024. Please visit our new website at and share your feedback with us!


EMBL-EBI provides programmatic access to various data resources and analysis tools via Web Services technologies 1) 2).

Web Services is an integration and inter-operation technology. To ensure client and server software from various sources will work well together, the technology is built on open standards:

For the transport layer Web Services utilise common network protocols, typically the Hypertext Transfer Protocol (HTTP) used to provide access to web sites.

Introduction to Web Services 

Sequence Analysis Tools Web Services

Sequence Analysis WS with Job Dispatcher


We kindly ask all users of EMBL-EBI Web Services to submit tool jobs in batches of no more than 30 at a time and not to submit more until the results and processing is complete. Please ensure that a valid email address is provided. Excessive usage of a particular resource will be dealt with in accordance with EMBL-EBI's Terms of Use. Please contact us if you need further information.

Chinese / 中文

敬告所有 EMBL-EBI Web Services 网络服务用户,请在呈交工具工作时,每批工作 不要超过30项,并请您在该批数据的结果和处理程序完成之后再呈交下一批工作。并请您提供一个有效的电子邮件地址。超额使用某项资源将按照 EMBL-EBI使用条款处理。 如需进一步信息,请通过此链接 与我们联系。

Korean / 한글

EMBL-EBI 웹서비스는 다량의 데이터(배치 등) 호출시, 사용자당 실시간으로 최대 30건의 처리를 제한적으로 사용하실 것을 권고드리고 있습니다. 보내신 건이 완료되기 전에, 재차 더 많은 데이터가 전송되지 않도록 협조 부탁드립니다. 또한 유효한 메일주소를 사용해주시기 바랍니다. 다량의 데이터 사용에 관한, EMBL-EBI 이용 약관을 준수해 주실것을 부탁드리며, 관련해 도움이 필요하시면, 문의 주시기 바랍니다.

Programs organised by function

Data RetrievalA list of EMBL-EBI Web Services for data retrieval.
Protein Functional AnalysisTools to perform protein functional analysis.
Sequence Similarity SearchIdentify potentially homologous sequences based on sequence similarity.
Multiple Sequence AlignmentAlignment of a set of three or more, protein or nucleotide sequences.
Pairwise Sequence AlignmentIdentify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences (protein or nucleic acid).
PhylogenyAccess to phylogenetic tree generation methods.
RNARNA Analysis
Sequence Format ConversionConvert between common sequence formats, or verify the formatting of a sequence.
Sequence StatisticsAnalyse a sequence to determine its properties and use statistics to assign significance.
Sequence Translation

Translate a coding nucleotide sequence in to a protein sequence, or back-translate from a protein sequence to a possible coding nucleotide sequence.

Sequence OperationsGenerate checksums for a sequence using a range of checksum/digest methods which can be used to identify potentially identical sequences when searching the corresponding checksum field in sequence databases such as those available from ENA and UniProt
Literature and OntologiesLook-up ontology terms and navigate ontology relationships or access and analyse the literature.


To cite the EMBL-EBI Job Dispatcher Web Services,  please refer to the following publication:

Madeira, F., Pearce, M., Tivey, ARN., Basutkar, P., Lee, J., Edbali, O., Madhusoodanan, N., Kolesnikov, A., Lopez, R. (2022).

Search and sequence analysis tools services from EMBL-EBI in 2022. Nucleic Acids Research, 2022; gkac240.

DOI: 10.1093/nar/gkac240

EuropePMC: 35412617