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8877 Search results for search term Chemical cross-linking coupled with mass spectrometry proteomics

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Spliceosome specifically associates with CTD phospho-serine 5 isoform of RNA polymerase II to promote co-transcriptional splicing
Species: Homo sapiens (Human)
Project description: that the mammalian spliceosome specifically associates with the S5P CTD isoform of RNA polymerase II (More)
Quantification method: Mass spectrometry
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-10-05
Quantitative cross-linking/mass spectrometry
Species: Homo sapiens (Human)
Project description: proteins. Second, standard quantitative proteomics software was not suitable for work with cross-linking (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2015-06-08
mitochondria cross-linking mass spectrometry
Species: Mus musculus (Mouse)
Project description: We performed cross-linking mass spectrometry experiments on intact mitochondria isolated from mouse (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-12-11
Cross-linking of the human MHC-I peptide-loading complex
Species: Homo sapiens (Human)
Project description: , followed by cross-linking mass spectrometry to aid EM structure elucidation. (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Modifications: methionine oxidation with neutral loss of 64 Da
Made public: 2017-11-07
Data independent acquisition cross-linking/mass spectrometry
Species: Gallus gallus (Chicken) Equus caballus (Horse) Homo sapiens (Human) Oryctolagus cuniculus (Rabbit) Bos taurus (Bovine)
Project description: Quantitative cross-linking/mass spectrometry (QCLMS) provides increasing structural detail on (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-01-25
A complimentary benzophenone cross-linking/mass spectrometry photochemistry
Species: Homo sapiens (Human)
Project description: -linker and show its successful application to cross-linking/mass spectrometry, by increasing data density (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-04-26
Optimized cross-linking mass spectrometry for in situ interaction proteomics
Species: Homo sapiens (Human)
Project description: spectral identification now permits large-scale cross-linking mass spectrometry (XL-MS). Here, we optimized (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-04-03
Cross-linking/mass spectrometry analysis of AUGMIN complex
Species: Drosophila melanogaster (Fruit fly)
Project description: We applied cross-linking/mass spectrometry to characterize in vivo Augmin from Drosophila in (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-04-04
Cross-linking/mass spectrometry analysis of the AOC complex
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: -linking/mass spectrometry has been applied to investigate interactions between Okp1/ Ame1 heterodimer (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-10-30
Quantitative cross-linking/mass spectrometry reveals subtle protein conformational changes
Species: Homo sapiens (Human)
Project description: We have developed quantitative cross-linking/mass spectrometry (QCLMS) to interrogate (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-06-16
A model dataset for quantitative cross-linking/mass spectrometry using isotope-labeled cross-linkers
Species: Homo sapiens (Human)
Project description: Dynamic proteins and multi-protein complexes govern most biological processes. Cross-linking/mass (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-05-06
Cross-linking mass spectrometric analysis of Ska-MT interactions
Species: Homo sapiens (Human)
Project description: Ska complex with MTs by cross-linking mass spectrometry (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-01-28
QToF Cross-linking Optimisation
Species: Bos taurus (Bovine)
Project description: Optimising the parameters for cross-link analysis using a QToF mass spectrometer. We performed (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-11-12
Sequential digestion with Trypsin and Elastase in cross-linking/mass spectrometry
Species: Homo sapiens (Human) Schizosaccharomyces pombe
Project description: Cross-linking/mass spectrometry has become an important approach for studying protein structures (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-03-07
Effects of a phosphomimetic mutation and nucleotide binding on the interaction of Hsp90a with co-chaperone Aha1 analyzed by chemical cross-linking with mass spectrometry
Species: Homo sapiens (Human)
Project description: ). Here we use chemical cross-linking with mass spectrometry to explore the the impacts of a (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-06-17
An integrated workflow for cross-linking/mass spectrometry
Species: Gallus gallus (Chicken) Equus caballus (Horse) Homo sapiens (Human) Chaetomium thermophilum Bos taurus (Bovine) Saccharomyces cerevisiae (Baker's yeast)
Project description: We present a concise workflow to enhance the mass spectrometric detection of cross-linked peptides (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-08-27
An optimised fragmentation regime for cross-linking/mass spectrometry
Species: Homo sapiens (Human)
Project description: ) CID, HCD, ETD, ETD with supplemental CID (ETciD) and ETD with supplemental HCD (EThcD) on cross-linked (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-08-02
Cross-Linking/mass spectrometry (CLMS) analysis of RNA polymerase II
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: The mass spectrometry data of a previous publication (Chen et.al. 2009 doi:10.1038/emboj.2009.401 (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-07-20
Chemical cross-linking mass spectrometry for systems structural biology in mouse heart tissue.
Species: Mus musculus (Mouse)
Project description: While modern structural biology technologies have greatly expand (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-11-30
A Simple Cross-Linking/Mass Spectrometry Workflow to Study System-Wide Protein Interactions in Drosophila Embryos
Species: Drosophila melanogaster (Fruit fly)
Project description: We provide a cross-linking/MS workflow that can be applied to complex systems. The software tool (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-07-09
Development of in planta chemical cross-linking-based quantitative interactomics in Arabidopsis
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: An in planta chemical cross-linking-based quantitative interactomics (IPQCX-MS) workflow has been (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-08-14
Cross-linking mass spectrometry of the CCAN and CCAN-Cenp-A complexes
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: Here we performed a cross-linking mass spectrometry analysis of the inner kinethocore complex CCAN (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-08-20
Identification of HJURP binding determinants on the Mis18 complex using photo-cross-linking and mass spectrometry
Species: Homo sapiens (Human)
Project description: performed UV-cross-linking and mass spectrometry (MS) experiments. (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-08-01
On the reproducibility of label-free quantitative cross-linking/mass spectrometry
Species: Homo sapiens (Human)
Project description: Quantitative cross-linking/mass spectrometry (QCLMS) is an emerging approach to study (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-01-02
Cross-linking of mouse laminin 111
Species: Mus musculus (Mouse)
Project description: matrix, contributing to tissue development and maintenance. We used cross-linking and mass spectrometry (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-12-22
Investigation of human TFIID subcomplexes 7TAF and 8TAF by cross-linking mass spectrometry
Species: Homo sapiens (Human)
Project description: 7TAF and 8TAF complexes have been cross-linked with the bifunctional reagent bis(sulfosuccinimidyl (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2015-07-20
Cross-linking/mass spectrometry for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
Species: Homo sapiens (Human) Escherichia coli Enterococcus faecalis (Streptococcus faecalis) Klebsiella pneumoniae 342
Project description: High-density cross-linking/mass spectrometry data was provided for four targets (T0957, T0968 (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-10-07
Quantitative cross-linking/mass spectrometry analysis revealed unknown structure of complement iC3
Species: Homo sapiens (Human)
Project description: We applied quantitative cross-linking/mass spectrometry (QCLMS) to interrogate the structure of iC3 (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-06-17
Cross-linking mass spectrometry analyses of three different kinetochore protein complexes (KMN, NDC80C, MIS12C) using an MS-cleavable cross-linker, BuUrBu (DSBU)
Species: Homo sapiens (Human)
Project description: In order to develop a simplified cross-linking mass spectrometry protocol, we applied one-step size (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-04-01
In planta chemical crosslinking and mass spectrometry analysis of protein structure and interaction in Arabidopsis
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: Mass spectrometry analysis in combination with the site-specific chemical cross-linking has emerged (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-06-28
Blind testing cross-linking/mass spectrometry under the auspices of the 11th critical assessment of methods of protein structure prediction (CASP11)
Species: Homo sapiens (Human) Ruminococcus gnavus Bacteroides caccae Staphylococcus aureus
Project description: provided high-density cross-linking/mass spectrometry data for four targets in CASP11, revealing to us some (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-12-02
Chemical cross-linking enables drafting ClpXP proximity maps and taking snapshots of in vivo interaction networks
Species: Homo sapiens (Human) Escherichia coli Staphylococcus aureus
Project description: combined chemical cross-linking and mass-spectrometry to capture transient binding interactions within ClpP (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-11-21
CASP XL-MS target H0957 (ID31): Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
Species: Escherichia coli
Project description: Chemical cross-linking coupled to mass spectrometry was used to provide distance restraints for (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-10-02
CASP XL-MS target H0953 (ID 51): Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
Species: Salmonella phage vB_SenMS16
Project description: Chemical cross-linking coupled to mass spectrometry was used to provide distance restraints for (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-10-02
CASP XL-MS target T0975: Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
Species: Homo sapiens (Human)
Project description: Chemical cross-linking coupled to mass spectrometry was used to provide distance restraints for (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-10-02
CASP XL-MS target T0985: Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
Species: Acholeplasma laidlawii (strain PG-8A)
Project description: Chemical cross-linking coupled to mass spectrometry was used to provide distance restraints for (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-10-02
CASP XL-MS target T0987: Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
Species: Enterococcus faecalis (Streptococcus faecalis)
Project description: Chemical cross-linking coupled to mass spectrometry was used to provide distance restraints for (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-10-02
CASP XL-MS target H0968 (ID32): Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
Species: Klebsiella pneumoniae 342
Project description: Chemical cross-linking coupled to mass spectrometry was used to provide distance restraints for (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-10-02
CASP XL-MS target T0981: Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
Species: Staphylococcus virus K
Project description: Chemical cross-linking coupled to mass spectrometry was used to provide distance restraints for (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-10-02
Identification of human PKD2 interaction partners from cytosolic protein fractions of HeLa cells by affinity enrichment cross-linking/MS
Species: Homo sapiens (Human)
Project description: subcellular protein fractions, using an affinity enrichment strategy combined with chemical cross-linking/mass (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-09-27
Identification of human PKD2 interaction partners from Golgi-enriched protein fractions of HeLa cells by affinity enrichment cross-linking/MS
Species: Homo sapiens (Human)
Project description: subcellular protein fractions, using an affinity enrichment strategy combined with chemical cross-linking/mass (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-09-27
Cross-linking Analysis (HSP70-HSP90-CHIP)
Species: Homo sapiens (Human)
Project description: combination of cryoelectron microscopy and crosslinking mass spectrometry, show the substrate located between (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-03-06
Quantitative cross-linking analysis of chemoresistant HeLa cells
Species: Homo sapiens (Human)
Project description: changes on a proteomic scale. In this project we combine SILAC with PIR cross-linking technology to (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2015-09-15
SIM-XL: a powerful and user-friendly tool for peptide cross-linking analysis
Species: Homo sapiens (Human)
Project description: Chemical cross-linking has emerged as a powerful approach for the structural characterization of (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2015-02-02
Chemical crosslinking mass spectrometry reveals the conformational landscape of the activation helix of PPARγ; a model for ligand-dependent antagonism
Species: Homo sapiens (Human)
Project description: -linking mass spectrometry. This study reveals a model of ligand-dependent antagonism of PPARγ by precisely (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-08-29
Cross-linking of the microtubule binding N-terminal domain of the companion of cellulose synthase 1 protein (CC1∆C223) from Arabidopsis thaliana with tubulin
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: In this study, we use Cross-linking Mass Spectrometry to identify the interaction sites of the (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-01-08
In culture cross-linking of bacterial cells reveals large scale dynamic protein-protein interactions at the peptide level.
Species: Bacillus subtilis subsp. subtilis str. 168
Project description: cross-linked peptides with less than a 1% false discovery rate by mass spectrometry and genome-wide data (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-05-23
Cross-linking MS data of CtNup82-Y complex
Species: Chaetomium thermophilum
Project description: XL-MS Data of the in vitro reconstituted CtNup82-Y complex. (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-10-17
Structural Analysis of a Prokaryotic Ribosome Using a Novel Amidinating Cross-Linker and Mass Spectrometry
Species: Escherichia coli
Project description: ) and mass spectrometry. Peptide cross-links derived from this complex structure were identified at high (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2015-12-23
3D topology of the SMC2/SMC4 sub-complex from chicken condensin I revealed by cross-linking and molecular modelling
Species: Gallus gallus (Chicken)
Project description: cryomicroscopic studies. Here we have used amino acid-selective cross-linking and mass spectrometry (CLMS (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2015-07-13
Non-covalently associated peptides are observed during liquid chromatography-mass spectrometry and affect cross-link analyses
Species: Gallus gallus (Chicken) Homo sapiens (Human) Bos taurus (Bovine)
Project description: Cross-Linking/mass spectrometry draws structural information out of covalently linked peptide pairs (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-01-25
Protein Structure Determination in Biological Matrices by Mass Spectrometry and Computational Biology
Species: Homo sapiens (Human)
Project description: a novel methodology that combines the use of this high-resolution cross-linking with conformational (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2015-09-22
In vivo cross-linking supports a head-to-tail mechanism for regulation of the plant plasma membrane P-type H+-ATPase
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: this study we used in vivo cross-linking experiments with a photo-reactive unnatural amino acid, p (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-09-24
Cross-Linked/Mass-spectrometry analysis of RNA-Polimerase II
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: The data of a previous publication (Chen et.al. 2009 doi:10.1038 (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-03-09
Molecular basis for RanGTP independent disassembly of an importin:ribosomal protein complex by the escortin Tsr2
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: Chemical cross-linking coupled to mass spectrometry was used to study the complex between the (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-09-10
CryoEM Structures of Arabidopsis DDR Complexes Involved in RNA-directed DNA Methylation
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: Chemical cross-linking coupled to mass spectrometry was used to study the A. thaliana DDR complex (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-07-15
Hybrid structural modeling of protein-RNA complexes using crosslinking of segmentally isotope labeled RNA and tandem mass spectrometry
Species: Homo sapiens (Human)
Project description: developed a new approach, termed cross-linking of segmentally isotope labeled RNA and tandem mass (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-03-27
Xilmass: a new approach towards the identification of cross-linked peptides
Species: Homo sapiens (Human)
Project description: Chemical cross-linking coupled with mass spectrometry plays an important role in unravelling (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-09-27
Development of an sulfoxide-based, CuAAC-enrichable cross-linker
Species: Bos taurus (Bovine)
Project description: Cross-linking of BSA with a novel cross-linker. Modification of the cross-linker containing (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-10-14
Apolipoprotein A1 forms 5/5 and 5/4 antiparallel dimers in human high-density lipoprotein
Species: Homo sapiens (Human)
Project description: We performed zero-order chemical cross-linking mass spectrometry experiments on reconstituted (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-04-09
Conformational control and DNA binding mechanism of the metazoan origin recognition complex
Species: Drosophila melanogaster (Fruit fly)
Project description: Chemical cross-linking coupled to mass spectrometry was used to study the architecture and (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-06-11
Neuropilin-2 (NRP2) is the receptor for human cytomegalovirus (HCMV) pentameric complex and is responsible for HCMV infection
Species: Homo sapiens (Human)
Project description: Chemical cross-linking coupled to mass spectrometry was used to study the complex between the HMCV (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-07-26
Crosslinking mass spectrometry of yeast Pol I pre-initiation complex
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: performed crosslinking mass spectrometry. (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-07-31
Efficient and robust proteome-wide approaches for crosslinking mass spectrometry
Species: Homo sapiens (Human)
Project description: Proteome-wide crosslinking of PC9 cell lysates with DSSO (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-06-15
Architecture and structural dynamics of the AROM complex
Species: Chaetomium thermophilum
Project description: Cross-linking/mass spectrometry was used to study inter-domain interactions in the multifunctional (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-10-02
Quantitative photo-crosslinking mass spectrometry reveals protein structure response to environmental changes
Species: Homo sapiens (Human) Bos taurus (Bovine)
Project description: conformational changes accessible to quantitative crosslinking mass spectrometry (QCLMS) approaches, crosslinking (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-07-01
In-Search Assignment of Monoisotopic Peaks Improves the Identification of Cross-Linked Peptides
Species: Homo sapiens (Human) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
Project description: Cross-linking/mass spectrometry has undergone a maturation process akin to standard proteomics by (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-04-05
Mango-EcoliBDP
Species: Escherichia coli
Project description: Dataset presented in Mango manuscript. Contains Ecoli cross-linked with BDP-NHP. Demonstration of (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-02-25
Optimizing the parameters governing the fragmentation of cross-linked peptides in a tribrid mass spectrometer
Species: Homo sapiens (Human)
Project description: optimized their collision energies with regard to optimal sequence coverage of cross-linked peptides. We (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-04-19
Mass spectrometric analysis of membrane protein topology
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: analysed by mass spectrometry. Dedicated software tools were used together with filtering steps optimized (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-10-11
Histone interaction landscapes visualized by crosslinking mass spectrometry in intact cell nuclei
Species: Homo sapiens (Human)
Project description: – collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-07-23
Optimized fragmentation improves the identification of peptides cross-linked using MS-cleavable reagents
Species: Equus caballus (Horse) Streptococcus pyogenes serotype M2 Cicer arietinum (Chickpea) (Garbanzo) Oryctolagus cuniculus (Rabbit) Homo sapiens (Human) Candida albicans (Yeast) Escherichia coli Bos taurus (Bovine)
Project description: Cross-linking mass spectrometry (XLMS) is becoming increasingly popular, and current advances are (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-02-01
Combining Affinity Enrichment, Cross-Linking with Photo-Amino Acids, and Mass Spectrometry for Probing Protein Kinase D2 Interactions
Species: Homo sapiens (Human)
Project description: analysis. Using HeLa cell lysates with photo-methionine and photo-leucine-labeled proteins, we were able to (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-04-10
Comprehensive identification of crosslinked peptides from methyltransferase Hmt1 and its substrate Npl3: use of multiple crosslinkers, mass spectrometry approaches and software platforms
Species: Escherichia coli Saccharomyces cerevisiae (Baker's yeast)
Project description: Chemical-Crosslinking Mass Spectrometry (XLMS) using MS-cleavable crosslinkers is fast becoming an (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-07-20
Integration of two in-depth proteomics approaches determines the high grade serous ovarian cancer-associated kallikrein-related peptidase 7 (KLK7) degradome
Species: Homo sapiens (Human)
Project description: Kallikrein-related peptidase 7 (KLK7) is a serine peptidase that (More)
Instrument: mass spectrometry
Experiment type: Shotgun proteomics
Made public: 2019-02-15
Molecular Mapping of FXIIIa Crosslinks
Species: Homo sapiens (Human)
Project description: detail regarding the specificity of FXIIIa cross-linking sites and substrates. (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-04-26
Human Mitochondrial NFS1-FXN XL LS-MS/MS
Species: Homo sapiens (Human)
Project description: disease. Using chemical cross-linking (XL) accompanied with LC-MS/MS, we studied the the interaction (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-07-11
Human Mitochondria NFS1-ISCU-FXN XL-MS
Species: Homo sapiens (Human)
Project description: disease. Using chemical cross-linking (XL) accompanied with LC-MS/MS, we studied the the interaction (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-07-11
The ubiquitin-like modifier FAT10 interferes with SUMO activation
Species: Homo sapiens (Human)
Project description: . Furthermore, quantitative chemical crosslinking coupled to mass spectrometry (q-XL-MS) was used to compare (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-10-08
Structural insights into the assembly and polyA signal recognition mechanism of the human CPSF complex
Species: Homo sapiens (Human)
Project description: , cross-linking MS, pull-downs and fluorescence polarisation assays. (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-12-18
Cryo-EM structure of the full human PRC2 and regulation by AEBP2 and JARID2
Species: Homo sapiens (Human)
Project description: Structural study of the PRC2 complex and its regulators AEPB2 and JARID2 combining cryo-EM, cross (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-01-25
Human BAF complex (HA-DPF2 pulldown and SS18-Flag pulldown)
Species: Homo sapiens (Human)
Project description: mammalian and Drosophila cells coupled with cross linking-mass spectrometry, we uncover three distinct and (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-10-18
Lamin A dimer and tetramer cross-links help explain disease mutations and polymer plasticity
Species: Homo sapiens (Human) Rattus norvegicus (Rat)
Project description: elusive. We employed cross-linking mass spectrometry to gain structural insights into lamin A dimer and (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-07-12
HeLa Cells Cross-Linked In Vivo versus as a Lysate
Species: Homo sapiens (Human)
Project description: HeLa Cells were cross-linked with PIR either in vivo or as a lysate. (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-07-12
Reconstitution of microtubule nucleation in vitro reveals novel roles for Mzt1
Species: Schizosaccharomyces pombe
Project description: -linking mass spectrometry using EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) cross-linker. Although (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-05-27
pLink 2: A high-speed search engine with systematic evaluation for proteome-scale identification of cross-linked peptides
Species: Escherichia coli
Project description: Crosslinking mass spectrometry (CXMS) is a powerful technique to study protein structures and (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-07-26
Clamping down on transcription: repression of archaeal RNAP by the novel viral regulator RIP
Species: Sulfolobus solfataricus
Project description: targets the host RNA polymerase and efficiently represses its activity. We have used cross-linking and (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-12-02
Molecular architecture of photoreceptor phosphodiesterase (PDE6) with activated G protein elucidates the mechanism of visual excitation
Species: Bos taurus (Bovine)
Project description: managing PDE6-associated retinal disease. To address this gap, here we used a cross-linking MS-based (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-11-11
In vivo protein interaction network analysis reveals porin-localized toxin inactivation in antibiotic resistant bacteria
Species: Acinetobacter baumannii AB5075
Project description: -linking and mass spectrometry, we identified 2,068 non-redundant cross-linked peptide pairs containing 245 (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-12-22
Dithiothreitol (DTT) acts as a specific, UV-inducible cross-linker in elucidation of protein-RNA interactions
Species: Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast)
Project description: In the present paper, we introduce dithiothreitol (DTT) as a potent protein-RNA cross-linker. To (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2015-10-13
Drosophila BAF complex crosslinking (
Species: Drosophila melanogaster (Fruit fly)
Project description: mammalian and Drosophila cells coupled with cross linking-mass spectrometry, we uncover three distinct and (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-10-18
Human pBAF complex (HA-BRD7, PHF10-HA pulldown)
Species: Homo sapiens (Human)
Project description: mammalian and Drosophila cells coupled with cross linking-mass spectrometry, we uncover three distinct and (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-10-18
Dam1 complex-microtubule interaction
Species: Candida albicans (Yeast)
Project description: we used cross-linking mass spectrometry to determine the molecular architecture of the Dam1 complex (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2016-03-01
Pre-40S ribosome biogenesis factor Tsr1 is an inactive structural mimic of translational GTPases
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: diffracted to 3.6 Å. Here we characterize both wild type Tsr1 and Tsr1ΔNΔloop using cross-linking/mass (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2017-02-08
Aurora B kinase disrupts the extended conformation of lattice-bound Kinesin-13 MCAK
Species: Homo sapiens (Human)
Project description: We reveal that MCAK has a compact conformation in solution using cross-linking and electron (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-01-07
Mechanism of pharmacochaperoning in ATP-sensitive potassium channels revealed by cryo-EM
Species: Rattus norvegicus (Rat)
Project description: , repaglinide, and carbamazepine. CyanurBiotinDimercaptoPropionylSuccinimide (CBDPS) cross-linking mass (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-08-12
Mechanism of pharmacochaperoning in ATP-sensitive potassium channels revealed by cryo-EM
Species: Rattus norvegicus (Rat)
Project description: , repaglinide, and carbamazepine. DSP cross-linking mass spectrometry was used to partially confirm cryoEM (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-11-12
XL-MS and Native MS for CSN-CRL2 complexes
Species: Homo sapiens (Human)
Project description: -electron microscopy (cryo-EM) with chemical cross-linking mass spectrometry (MS). These structures reveal a (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2019-09-13
Comprehensive XL-MS reveals parallel orientation and versatile conformations of plant HOP2/MND1
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: relation of the complex partners and their domains in solution. We performed chemical cross-linking in (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2015-11-05
Human mitochondrial cysteine desulfurase complex
Species: Homo sapiens (Human)
Project description: : NFS1, ISD11 and acyl carrier protein (ACP). In this study we used chemical crosslinking coupled with (More)
Experiment type: Chemical cross-linking coupled with mass spectrometry proteomics
Made public: 2018-07-11

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