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6356 Search results for search term Affinity purification coupled with mass spectrometry proteomics

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Spliceosome specifically associates with CTD phospho-serine 5 isoform of RNA polymerase II to promote co-transcriptional splicing
Species: Homo sapiens (Human)
Project description: that the mammalian spliceosome specifically associates with the S5P CTD isoform of RNA polymerase II (More)
Quantification method: Mass spectrometry
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-10-05
YLR455 affinity purification
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: These studies define a new form of the NuA3 complex that associates with H3K36me3 to effect (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2014-08-14
Lactate racemase proteins MALDI TOF/TOF
Species: Lactobacillus plantarum
Project description: This project reports the characterization of the lactate racemas (More)
Instrument: 4800 Proteomics Analyzer
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2014-04-02
A novel inducible retroviral expression system for StrepHA-tandem affinity purification mass-spectrometry-based proteomics
Species: Homo sapiens (Human) Mus musculus (Mouse)
Project description: Tandem-affinity purification mass spectrometry using the streptavidin-hemagglutinin (SH)-tag has (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2016-01-05
CD147 mutants affinity purification mass spectrometry from human T cells
Species: Homo sapiens (Human)
Project description: . Using affinity purification mass spectrometry (AP-MS) of HA-tagged CD147 swap and deletion mutants, we (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2016-01-28
iMixPro: intelligent Mixing of Proteomes for elimination of false positives in affinity purification-mass spectrometry
Species: Homo sapiens (Human)
Project description: . Different strategies currently exist to analyze such protein complexes by mass spectrometry, including (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2016-09-27
Tandem affinity purification of histones, coupled to mass spectrometry, identifies new sites of post-translational modification and associated proteins in Saccharomyces cerevisiae
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: Our results can be considered as a starting point to gain unders (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2016-01-28
Highly accurate protein complex retrieval by affinity enrichment MS rather than affinity purification MS
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: purification coupled to mass spectrometry (AP-MS) is one of the most promising methods for their investigation (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2015-03-26
Identifying the interacting proteins of lignin biosynthetic P450 enzymes by affinity purification coupled with LC-MS/MS analysis
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: enzymes by affinity purification and LC-MS/MS analysis. To this end, the genomic pieces of three lignin (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-05-02
Comprehensive Immunoproteome of Mycoplasma bovis
Species: Mycoplasma bovis HB0801 Bos taurus (Bovine)
Project description: This study was aimed to elucidate a global antigenic profile of Mycoplasma bovis (M. bovis) with (More)
Instrument: 4800 Proteomics Analyzer
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2016-06-13
Tandem affinity purification and Mass spectrometry (TAP-MS) analysis of PCH1’s associated proteins in various Arabidopsis genetic backgrounds.
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: overexpressed or under the control of its own promoter). By combining TAP-MS with genetics, we investigate the (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2016-02-09
Evaluation of a bead-free immunoprecipitation technique coupled with tandem mass spectrometry for identification of plant-virus protein interactions (DeBlasio_JBT_2017)
Species: Nicotiana benthamiana
Project description: proteins that interact with wild-type (WT) and PLRV mutant form. Comparison of host and viral proteins (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-10-24
Quantitative tandem affinity purification, an effective tool to investigate protein complex composition in plant hormone signaling: strigolactones in the spotlight
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: affinity purification coupled to mass spectrometry (TAP-MS) is combined with label-free quantification (LFQ (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-04-26
Identification of thioredoxin targets in Chlamydomonas reinhardtii using an affinity purification approach
Species: Chlamydomonas reinhardtii
Project description: photosynthetic model organism. Using an affinity purification strategy, we identified 980 thioredoxin targets (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2017-07-28
Identification of PWWP2A interactors using label-free quantitative affinity enrichment mass spectrometry
Species: Homo sapiens (Human)
Project description: performed pulldowns using PWWP2A as a bait protein. Label-free quantitative mass spectrometry-based (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-08-30
Identification of inositol 1,4,5-trisphosphate-binding proteins by heparin-agarose affinity purification and LTQ ORBITRAP MS in Oryza sativa
Species: Rice(O. sativa)
Project description: affinity purification. The IP3 binding activities of these protein fractions were determined by [3H]IP3 (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2014-09-25
Quantitative proteomics of affinity purified yeast NMD complexes
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: affinity purification and mass-spectrometry with 7 different NMD-related factors, with or without Rnase, in (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-03-12
Mass spectrometry analysis of proteins interacting with STAT3
Species: Homo sapiens (Human)
Project description: Mass spectrometry analysis of proteins interacting with STAT3¬Flag was performed. STAT3-Flag was (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-08-29
Pseudomonas aeruginosa PA3904 pull down coupled to LC-MS/MS
Species: Pseudomonas aeruginosa PAO1
Project description: was mixed with SDS-loading buffer, boiled and run on a 12% SDS-PAGE for 0.6 cm.  After coomassie (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-03-09
Identification of protein-protein interactions involving the eIF4E binding proteins Caf20p and Eap1p on yeast via affinity purification and LC-MSMS.
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: label-free mass spectrometry to compare the proteomes pulled-down with the TAP-tagged 4E-BPs, and the (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2016-06-29
xIP-MS: topological analysis of chromatin-associated protein complexes using single affinity purification
Species: Homo sapiens (Human)
Project description: In recent years, cross-linking mass spectrometry (XL-MS) has proven to be a robust and effective (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2015-11-17
Global profiling of protein-DNA and protein-nucleosome binding affinities using quantitative mass spectrometry
Species: Homo sapiens (Human)
Project description: affinity purifications from nuclear extracts coupled with isobaric chemical labeling and mass spectrometry (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-05-02
Mass spectrometry identification of Trypanosoma brucei bloodstream form proteins that interacts with Ins(1,4,5)P3 and Ins(1,3,4,5)P4.
Species: Trypanosoma brucei
Project description: Mass spectrometry identification of Trypanosoma brucei bloodstream form proteins that interacts (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-10-26
Isolation of PfPuf3 protein complex and mass spectrometry (MS) analysis
Species: Plasmodium falciparum UGK 730.2
Project description: yeast Puf6. Affinity purification of a C-terminal PTP-tagged variant of PfPuf3 revealed an association (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-03-12
Arabidopsis endomembrane enrichments: Affinity enrichment of RABF2b/RFP-ARA7
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2015-04-22
Arabidopsis endomembrane enrichments: Affinity enrichment of YFP-RABG3f
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2015-04-22
Arabidopsis endomembrane enrichments: Affinity enrichment ofYFP-RABD2a/ARA5
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2015-04-22
Arabidopsis endomembrane enrichments: Affinity enrichment of mCherry bait
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2015-04-22
Arabidopsis endomembrane enrichments: Affinity enrichment of GFP bait
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2015-04-22
Arabidopsis endomembrane enrichments: Affinity enrichment of RABF1/ARA6-RFP
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2015-04-22
Arabidopsis endomembrane enrichments: Affinity enrichment of YFP-VAMP711
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2015-04-22
Arabidopsis endomembrane enrichments: Affinity enrichment of YFP-GOT1
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2015-04-22
Affinity capture of S. cerevisiae protein after glutaraldehyde stabilization
Species: Saccharomyces cerevisiae (Baker's yeast)
Project description: information are those based on affinity capture of target bait proteins in combination with mass spectrometric (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-10-19
Mass spectrometry analysis of the Themis1 interactome in murine thymocytes
Species: Mus musculus (Mouse)
Project description: proteomic and TCR signaling reporter mice to gain insight into Themis1 signaling function. Mass spectrometry (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2017-02-06
Characterization of the PAK4 interactome by quantitative mass spectrometry
Species: Homo sapiens (Human)
Project description: p21-activated kinase 4 (PAK4) is a serine/threonine kinase critical during development and with a (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-10-27
Dissection of affinity captured LINE-1 macromolecular complexes
Species: Homo sapiens (Human)
Project description: differential affinity purifications, quantitative mass spectrometry, and next generation RNA sequencing, we (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-01-17
Identification of In Vivo HLA-DR-Presented Self-Peptides (T Cell Epitopes) in Synovial Tissue, Synovial Fluid and Peripheral Blood in Patients with Rheumatoid Arthritis or Lyme Arthritis
Species: Homo sapiens (Human)
Project description: mononuclear cells (SF), and peripheral blood mononuclear cells (PB) from five patients with RA and eight with (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Modifications: cysteinylation (disulfide with free L-cysteine)
Made public: 2016-10-13
Identification of GLI-associated proteins by mass spectrometry
Species: Mus musculus (Mouse)
Project description: were identified in the complex by subsequent mass spectrometry analysis. (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2016-02-04
Methylated-antibody affinity purification to improve proteomic identification of plant RNA polymerase Pol V complex and the interacting proteins
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: Affinity purification followed by enzymatic digestion and mass spectrometry has been widely (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2017-02-28
Identification of Drosophila centromere associated proteins
Species: Drosophila melanogaster (Fruit fly)
Project description: affinity purification-MS analysis of Drosophila centromeric chromatin (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2014-07-31
Arabidopsis endomembrane enrichments: Affinity enrichment of CLC2-GFP in WS-2
Species: Arabidopsis thaliana (Mouse-ear cress)
Project description: protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2015-04-22
Affinify Purification of EZH2 and phosphodeficient EZH2 identifies phosphorylation dependent interactions of EZH2
Species: Homo sapiens (Human)
Project description: significantly associated with ER- disease and low H3K27me3 levels. Here, we explore the interactome of EZH2 and (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-07-03
The EML4-ALK Interactome in Lung Cancer
Species: Homo sapiens (Human)
Project description: and protein complex annotation using affinity purification coupled with mass spectrometry and further (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-10-26
Comprehensive characterization of Minichromosome Maintenance Complex (MCM) protein interactions using affinity and proximity purifications coupled to mass spectrometry.
Species: Homo sapiens (Human)
Project description: This project is the complete interactome of the Minichromosome M (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2016-08-09
SILAC Mass Spectrometry of U2AF2 Interactome in Resting and Activated CD4 T cells
Species: Homo sapiens (Human)
Project description: differentially expressed and/or alternatively spliced during activation. Using RIP mass spectrometry, a unique (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2016-04-27
Myosin VIIA 138-1 immunoaffinity purification
Species: Gallus gallus (Chicken)
Project description: shotgun and targeted mass spectrometry, we demonstrated that MYO7A forms a complex with PDZD7, a paralog (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2016-08-16
Proximity labeling and identification of the proteins that interact with Siglec-14 on THP-1 cells
Species: Homo sapiens (Human)
Project description: streptavidin affinity purification and proteomic analysis. MaxQuant software was used for the quantitative (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-11-04
14-3-3 affinity capture to identify new targets of 14-3-3 signaling during dsRNA-stimulation in human keratinocytes.
Species: Homo sapiens (Human)
Project description: We have combined two proteomic approaches, phosphoproteomics and 14-3-3 affinity capture, to (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2014-08-05
Bod1 interactome in mitotic HeLa cells
Species: Homo sapiens (Human)
Project description: combined affinity purification of Bod1 with label-free quantitative mass spectrometry. (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2018-10-24
Urea artifacts interfere with immunopurification of lysine acetylation
Species: Homo sapiens (Human)
Project description: purification. We propose an alternative strategy using an ionic detergent which improves significantly the (More)
Experiment type: Affinity purification coupled with mass spectrometry proteomics
Made public: 2017-01-11

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