Project PXD013503

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Summary

Title

Phosphoproteomic analysis of estrogen signaling upstream and downstream of mTORC1

Description

Phosphoproteomic data from human MCF-7 cells treated with estrogen only or estrogen plus rapamycin, an mTOR inhibitor.

Sample Processing Protocol

Samples were digested with trypsin, then analyzed by LC-MS/MS

Data Processing Protocol

Data was searched with MSGF+ using PNNL's DMS Processing pipeline

Contact

Matthew Monroe, Pacific Northwest National Laboratory
Marina K. Holz, New York Medical College ( lab head )

Submission Date

15/04/2019

Publication Date

08/08/2019

Disease

breast cancer

Quantification

Not available

Experiment Type

Shotgun proteomics

Assay count

6

Publication

    Cuesta R, Gritsenko MA, Petyuk VA, Shukla AK, Tsai CF, Liu T, McDermott JE, Holz MK. Phosphoproteome Analysis Reveals Estrogen-ER Pathway as a Modulator of mTOR Activity Via DEPTOR. Mol Cell Proteomics. 2019 18(8):1607-1618 PubMed: 31189691

Assay

Showing 1 - 6 of 6 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 108412 Holz_MCF7_TMT_PP_01_17Nov17_Elm_AQ-17-10-03_msgfplus.mzid 31951 61763 33024 41827 0
2 108413 Holz_MCF7_TMT_PP_02_17Nov17_Elm_AQ-17-10-03_msgfplus.mzid 34633 74365 39742 50222 0
3 108414 Holz_MCF7_TMT_PP_03_17Nov17_Elm_AQ-17-10-03_msgfplus.mzid 35355 74409 39927 50133 0
4 108415 Holz_MCF7_TMT_PP_04_17Nov17_Elm_AQ-17-10-03_msgfplus.mzid 34622 70833 38372 48515 0
5 108411 Holz_MCF7_TMT_PP_05_17Nov17_Elm_AQ-17-10-03_msgfplus.mzid 35229 74432 39884 51954 0
6 108416 Holz_MCF7_TMT_PP_06_17Nov17_Elm_AQ-17-10-03_msgfplus.mzid 34797 74814 39284 50715 0