Project PXD013406

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Summary

Title

Photosystem II assembly No-reaction center (NRC) complex

Description

LC-MS/MS identification of small subunit peptides of Photosystem II assembly intermediate (NRC) with/without trypsin digestion. Peptide sequence identification with HCD fragmentation using Q-Exactive plus mass spectrometer (Thermo-Fisher)

Sample Processing Protocol

See materials and methods, briefly, PSII sample were cleaned up by using 2D clean up kit and resuspended in 70% acetone, 19% water, 10% isopropanol, 1% formic acid (v/v). Trpsin digestion of in-solution protein or excised gel band followed lab routine.

Data Processing Protocol

PMI (Protein Metrics), Bionic, Xcalibre qual brower in combination with ProteinProspector and Proteomics Toolkit (http://db.systemsbiology.net:8080/proteomicsToolkit/FragIonServlet.html)

Contact

Haijun Liu, Washington University in St. Louis
Himadri B Pakrasi, Biology,Washington University in St. Louis ( lab head )

Submission Date

08/04/2019

Publication Date

14/10/2019

Publication

    Weisz DA, Johnson VM, Niedzwiedzki DM, Shinn MK, Liu H, Klitzke CF, Gross ML, Blankenship RE, Lohman TM, Pakrasi HB. A novel chlorophyll protein complex in the repair cycle of photosystem II. Proc Natl Acad Sci U S A. 2019 PubMed: 31594847

Assay

Showing 1 - 9 of 9 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 108287 20190405_NRC_47.raw_Haijun_Liu.mzid 6 1415 6 7997 0
2 108288 20190405_NRC_dO.raw_Haijun_Liu.mzid 6 647 6 7561 0
3 108289 20190405_PS2M_1.raw.mzid 8 3046 9 7945 0
4 108283 47_NRC.raw_20190405_Haijun.mzid 15 461 103 14136 0
5 108284 47_sub_M.raw_20190405_Haijun.mzid 42 790 145 15731 0
6 108285 dO_M.raw_20190405_Haijun.mzid 35 2286 298 16194 0
7 108286 dO_NRC.raw_20190406_Haijun.mzid 25 894 149 16149 0
8 108290 dO_subM.raw_20190406_Haijun.mzid 30 1076 197 15903 0
9 108282 dO_NRC_soln.raw_20190406_Haijun.mzid 61 1038 212 21724 0