Project PXD011562

Summary

Title

Yeast mirror LC-MS/MS

Description

Precision de novo peptide sequencing using mirror proteases of Ac-LysargiNase and trypsin for large-scale proteomics

Sample Processing Protocol

Trypsin and Ac-LysargiNase to split the Yeast samples

Data Processing Protocol

Database search engine: pFind Precursor/Fragment mass tolerance: 20 ppm/20 ppm De novo sequencing: pNovoM and pNovo+ Precursor/Fragment mass tolerance: 20 ppm/20 ppm PEAKS Precursor/Fragment mass tolerance: 20 ppm/0.02Da

Contact

Xi Wang, pFind
Ping Xu, State Key Laboratory of Proteomics, National Center for Protein Sciences Beijing, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China ( lab head )

Submission Date

01/11/2018

Publication Date

11/01/2019

Instrument

LTQ Orbitrap Velos

Software

Not available

Experiment Type

Shotgun proteomics

Publication

    Yang H, Li Y, Zhao M, Wu F, Wang X, Xiao W, Wang Y, Zhang J, Wang F, Xu F, Zeng WF, Overall CM, He SM, Chi H, Xu P. Precision de novo peptide sequencing using mirror proteases of Ac-LysargiNase and trypsin for large-scale proteomics. Mol Cell Proteomics. 2019 PubMed: 30622160