Project PXD010468

PRIDE Assigned Tags:
Biological Dataset
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Summary

Title

CHD1_RIME

Description

Crosslinked chromatin bound protein complexes were immunopurified, digested, and analyzed by mass spectrometry to identify and quantify proteins associate with the chromodomain helicase (Chd1).

Sample Processing Protocol

Samples were processed using the RIME method (PMID:26797456). Samples were formaldehyde cross-linked prior to immunoprecipitation and on-bead digestion. Desalted peptides were analyzed by LC-MS/MS on an Orbitrap Fusion Mass Spectrometer.

Data Processing Protocol

Peptide spectra were searched with SEQUEST against a concatenated database containing all human proteins and the reversed sequences. Peptides were filtered to 1% FDR using the target-decoy approach. Label-free area under the curve quantification was performed to assess protein levels relative to controls.

Contact

Noah Dephoure, Weill Cornell Medical College Department of Biochemistry
Noah Dephoure, Weill Cornell Medical College ( lab head )

Submission Date

18/07/2018

Publication Date

13/08/2019

Tissue

Not available

Instrument

Orbitrap Fusion

Software

Not available

Modification

Not available

Publication

    Augello MA, Liu D, Deonarine LD, Robinson BD, Huang D, Stelloo S, Blattner M, Doane AS, Wong EWP, Chen Y, Rubin MA, Beltran H, Elemento O, Bergman AM, Zwart W, Sboner A, Dephoure N, Barbieri CE. CHD1 Loss Alters AR Binding at Lineage-Specific Enhancers and Modulates Distinct Transcriptional Programs to Drive Prostate Tumorigenesis. Cancer Cell. 2019 35(4):603-617.e8 PubMed: 30930119