proteomic analysis of gamma-tocotienol(GT3)-treated human diploid fibroblasts (HDFs)
the proteomes of untreated and GT3-treated HDFs were compared
Sample Processing Protocol
whole protein was extracted with ripa in-gel. 1 sample divided into 6 faction. Subjced to LCMS/MS run.
Data Processing Protocol
proteins were identified and quantified by MaxQuant, DEPs were identifeid by Perseus, Gene ontology for DEPs was annotated by Panther Classification System.
Tan JK, Jaafar F, Makpol S. Proteomic profiling of senescent human diploid fibroblasts treated with gamma-tocotrienol. BMC Complement Altern Med. 2018 18(1):314 PubMed: 30497457