Project PXD009342

PRIDE Assigned Tags:
Technical Dataset



Accurate, Sensitive, and Precise Multiplexed Proteomics using the Complement Reporter Ion Cluster


Quantitative analysis of proteomes across multiple time points, organelles, and perturbations is essential for understanding both fundamental biology and disease states. The development of isobaric tags (e.g. TMT) have enabled the simultaneous measurement of peptide abundances across several different conditions. These multiplexed approaches are promising in principle because of advantages in throughput and measurement quality. However, in practice existing multiplexing approaches suffer from key limitations. In its simple implementation (TMT-MS2), measurements are distorted by chemical noise leading to poor measurement accuracy. The current state-of-the-art (TMT-MS3) addresses this, but requires specialized quadrupole-iontrap-Orbitrap instrumentation. The complement reporter ion approach (TMTc) produces high accuracy measurements and is compatible with many more instruments, like quadrupole-Orbitraps. However, the required deconvolution of the TMTc cluster leads to poor measurement precision. Here, we introduce TMTc+, which adds the modeling of the MS2-isolation step into the deconvolution algorithm. The resulting measurements are comparable in precision to TMT-MS3/MS2. The improved duty cycle, and lower filtering requirements make TMTc+ more sensitive than TMT-MS3 and comparable with TMT-MS2. At the same time, unlike TMT-MS2, TMTc+ is exquisitely able to distinguish signal from chemical noise even outperforming TMT-MS3. Lastly, we compare TMTc+ to quantitative label-free proteomics of total HeLa lysate and find that TMTc+ quantifies 7.8k versus 3.9k proteins in a 5-plex sample. At the same time the median coefficient of variation improves from 13% to 4%. Thus, TMTc+ advances quantitative proteomics by enabling accurate, sensitive, and precise multiplexed experiments on more commonly used instruments.

Sample Processing Protocol

Samples were prepared using standard TMT processing protocol. Protein was extracted with a chloroform/methanol precipitation, proteins were re-suspended in guanidine hydrochloride and digested with trypsin and LysC. Peptides were tagged with TMT reagents, combined, de-salted with a C18 column and analyzed by LC-MS.

Data Processing Protocol

GFY was licensed from the Gygi lab at Harvard University to perform ReadW on raw files, sequests searches, and control peptide and protein FDR rates. TMTc+ reporter ions were deconvolved offline using a custom matlab script which is available to all academics as a wrapped upon request. Label free samples were analyzed in MaxQuant.


Matthew Sonnett, Princeton University
Martin Wühr, Department of Molecular Biology and the Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA, 08540 ( lab head )

Submission Date


Publication Date



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Experiment Type

Shotgun proteomics


    Sonnett M, Yeung E, Wühr M. Accurate, Sensitive, and Precise Multiplexed Proteomics using the Complement Reporter Ion Cluster. Anal Chem. 2018 PubMed: 29522331