Project PXD008688

PRIDE Assigned Tags:
Technical Dataset

Summary

Title

E. coli mirror LC-MS/MS

Description

Precision de novo peptide sequencing using mirror proteases of Ac-LysargiNase and trypsin for large-scale proteomicsPrecision de novo peptide sequencing using mirror proteases of Ac-LysargiNase and trypsin for large-scale proteomics

Sample Processing Protocol

Trypsin and Ac-LysargiNase to split the E. coli samples

Data Processing Protocol

Database search engine: pFind Precursor/Fragment mass tolerance: 20 ppm/20 ppm De novo sequencing: pNovoM, pNovo+ and PEAKS Precursor/Fragment mass tolerance: 20 ppm/20 ppm

Contact

Hao Yang, Key Lab of Intelligent Information Processing of Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS, University of Chinese Academy of Sciences
Ping Xu, State Key Laboratory of Proteomics, National Center for Protein Sciences Beijing, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China ( lab head )

Submission Date

16/01/2018

Publication Date

11/01/2019

Tissue

Not available

Instrument

LTQ Orbitrap Velos

Software

Not available

Quantification

Not available

Experiment Type

Shotgun proteomics

Publication

    Yang H, Li Y, Zhao M, Wu F, Wang X, Xiao W, Wang Y, Zhang J, Wang F, Xu F, Zeng WF, Overall CM, He SM, Chi H, Xu P. Precision de novo peptide sequencing using mirror proteases of Ac-LysargiNase and trypsin for large-scale proteomics. Mol Cell Proteomics. 2019 PubMed: 30622160