Project PXD008638

PRIDE Assigned Tags:
Technical Dataset Biomedical Dataset

Summary

Title

Thermal proteome profiling of JQ1

Description

Goal of this experiment was the identification of proteins affected in their thermal stability by JQ1.

Sample Processing Protocol

Treatment of HepG2 cells with Vehicle or JQ1 followed by subsequent heat treatment. Lysis of cells in PBS with 0.8% IGEPAL CA-630. Labeling of cells subjected to different temperatures with TMT and subsequent mass spectrometric analysis.

Data Processing Protocol

Data analysis was performed using an in-house pipeline and searched with Mascot 2.4

Contact

Maria Faelth Savitski, Cellzome
Marcus Bantscheff, Cellzome ( lab head )

Submission Date

09/01/2018

Publication Date

02/05/2018

Publication

    Savitski MM, Zinn N, Faelth-Savitski M, Poeckel D, Gade S, Becher I, Muelbaier M, Wagner AJ, Strohmer K, Werner T, Melchert S, Petretich M, Rutkowska A, Vappiani J, Franken H, Steidel M, Sweetman GM, Gilan O, Lam EYN, Dawson MA, Prinjha RK, Grandi P, Bergamini G, Bantscheff M. Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis. Cell. 2018 173(1):260-274.e25 PubMed: 29551266