Project PXD008547

PRIDE Assigned Tags:
Biological Dataset
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Summary

Title

Lymnaea shell proteomics

Description

The calcifying matrix proteins embedded within the shell biomineral structure is a mixture of proteins, glycoproteins, and polysaccharides that are involved in the shell formation processes. Here with develop bottom-up shotgun proteomic investigation of these proteins, thanks to specific transcriptomic data generated from the calcifying mantle of adult Lymnaea.

Sample Processing Protocol

The acid-soluble and the acid-insoluble matrix proteins were extracted from adult shells, then reduced, alkyled and digested with trysin prior to nanoLC-ESI MS/MS analysis on a QSTAR (Sciex) coupled to a Dionex U3000 HPLC.

Data Processing Protocol

MFG file was then generated from wiff raw data using Anatyst 1.1 software, then data were analyzed using Mascot serach engine 2.4, against an in-house transcriptomic database.

Contact

Benjamin Marie, CNRS
Benjamin MARIE, MNHN / CNRS ( lab head )

Submission Date

22/12/2017

Publication Date

05/04/2018

Tissue

Not available

Instrument

QSTAR

Software

Not available

Quantification

Not available

Experiment Type

Shotgun proteomics

Assay count

1

Publication

    Herlitze I, Marie B, Marin F, Jackson DJ. Molecular modularity and asymmetry of the molluscan mantle revealed by a gene expression atlas. Gigascience. 2018 PubMed: 29788257

Assay

Showing 1 - 1 of 1 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 83157 All_Lymnaea.mzid 76 875 158 1230 0