PRIDE Assigned Tags:Biological Dataset
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Lymnaea shell proteomics
The calcifying matrix proteins embedded within the shell biomineral structure is a mixture of proteins, glycoproteins, and polysaccharides that are involved in the shell formation processes. Here with develop bottom-up shotgun proteomic investigation of these proteins, thanks to specific transcriptomic data generated from the calcifying mantle of adult Lymnaea.
Sample Processing Protocol
The acid-soluble and the acid-insoluble matrix proteins were extracted from adult shells, then reduced, alkyled and digested with trysin prior to nanoLC-ESI MS/MS analysis on a QSTAR (Sciex) coupled to a Dionex U3000 HPLC.
Data Processing Protocol
MFG file was then generated from wiff raw data using Anatyst 1.1 software, then data were analyzed using Mascot serach engine 2.4, against an in-house transcriptomic database.
Herlitze I, Marie B, Marin F, Jackson DJ. Molecular modularity and asymmetry of the molluscan mantle revealed by a gene expression atlas. Gigascience. 2018 PubMed: 29788257
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