Project PXD006367

PRIDE Assigned Tags:
Biological Dataset
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Summary

Title

Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education

Description

In a cross-sectional approach, we analyzed the influence of age, sex, body mass index (BMI), smoking, and education on salivary protein signatures in whole saliva samples of 187 individuals. Subjects were randomly selected from the population-based Study of Health in Pomerania (SHIP-Trend).

Sample Processing Protocol

- sample collection with Salivette (Sarstedt, Nümbrecht, Germany) - TCA precipitation of proteins - tryptic digestion - desalting of digests with ZipTip µC-18 - data dependent LC-MS/MS measurement on nano-UPLC/LTQ Orbitrap Velos

Data Processing Protocol

- data processing via Genedata Expressionist software package - identification via Mascot algorithm

Contact

Annette Murr, University Medicine Greifswald
Uwe Völker, Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany ( lab head )

Submission Date

24/04/2017

Publication Date

16/05/2017

Tissue

saliva

Instrument

LTQ Orbitrap Velos

Software

Not available

Quantification

Not available

Experiment Type

Shotgun proteomics

Assay count

209

Publication

    Murr A, Pink C, Hammer E, Michalik S, Dhople VM, Holtfreter B, Völker U, Kocher T, Gesell Salazar M. Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education. J Proteome Res. 2017 May 8 PubMed: 28481548

Assay

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Showing 31 - 40 of 209 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
31 76165 12_batch2.dat-pride-filtered.xml 902 5538 2171 16734 0
32 76045 130_batch7.dat-pride-filtered.xml 1104 5265 2655 17205 0
33 76166 131_batch7.dat-pride-filtered.xml 930 4210 2078 16494 0
34 76046 132_batch6.dat-pride-filtered.xml 1097 4936 2593 17254 0
35 76167 133_batch6.dat-pride-filtered.xml 786 3308 1727 15221 0
36 76047 134_batch6.dat-pride-filtered.xml 920 4234 2161 14851 0
37 76168 135_batch6.dat-pride-filtered.xml 368 1364 725 12394 0
38 76048 136_batch6.dat-pride-filtered.xml 850 3997 2063 16201 0
39 76169 137_batch6.dat-pride-filtered.xml 913 4201 2071 16415 0
40 76049 138_batch6.dat-pride-filtered.xml 1016 4437 2409 17182 0