Project PXD006367

PRIDE Assigned Tags:
Biological Dataset
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Summary

Title

Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education

Description

In a cross-sectional approach, we analyzed the influence of age, sex, body mass index (BMI), smoking, and education on salivary protein signatures in whole saliva samples of 187 individuals. Subjects were randomly selected from the population-based Study of Health in Pomerania (SHIP-Trend).

Sample Processing Protocol

- sample collection with Salivette (Sarstedt, Nümbrecht, Germany) - TCA precipitation of proteins - tryptic digestion - desalting of digests with ZipTip µC-18 - data dependent LC-MS/MS measurement on nano-UPLC/LTQ Orbitrap Velos

Data Processing Protocol

- data processing via Genedata Expressionist software package - identification via Mascot algorithm

Contact

Annette Murr, University Medicine Greifswald
Uwe Völker, Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany ( lab head )

Submission Date

24/04/2017

Publication Date

16/05/2017

Tissue

saliva

Instrument

LTQ Orbitrap Velos

Software

Not available

Quantification

Not available

Experiment Type

Shotgun proteomics

Assay count

209

Publication

    Murr A, Pink C, Hammer E, Michalik S, Dhople VM, Holtfreter B, Völker U, Kocher T, Gesell Salazar M. Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education. J Proteome Res. 2017 May 8 PubMed: 28481548

Assay

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Showing 201 - 209 of 209 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
201 76115 GS_batch2.dat-pride-filtered.xml 916 4452 2143 15392 0
202 76236 GS_batch3.dat-pride-filtered.xml 939 4362 2294 15575 0
203 76116 GS_batch4.dat-pride-filtered.xml 939 4215 2253 16201 0
204 76237 GS_batch5.dat-pride-filtered.xml 975 4335 2315 15961 0
205 76117 GS_batch6.dat-pride-filtered.xml 981 4211 2301 15778 0
206 76238 GS_batch7.dat-pride-filtered.xml 1061 4497 2506 15931 0
207 76118 GS_batch8.dat-pride-filtered.xml 1078 4590 2536 15826 0
208 76239 GS_batch9.dat-pride-filtered.xml 1056 4368 2485 15573 0
209 76119 GS_batchR.dat-pride-filtered.xml 790 3633 1815 15979 0