Project PXD006367

PRIDE Assigned Tags:
Biological Dataset
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Summary

Title

Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education

Description

In a cross-sectional approach, we analyzed the influence of age, sex, body mass index (BMI), smoking, and education on salivary protein signatures in whole saliva samples of 187 individuals. Subjects were randomly selected from the population-based Study of Health in Pomerania (SHIP-Trend).

Sample Processing Protocol

- sample collection with Salivette (Sarstedt, Nümbrecht, Germany) - TCA precipitation of proteins - tryptic digestion - desalting of digests with ZipTip µC-18 - data dependent LC-MS/MS measurement on nano-UPLC/LTQ Orbitrap Velos

Data Processing Protocol

- data processing via Genedata Expressionist software package - identification via Mascot algorithm

Contact

Annette Murr, University Medicine Greifswald
Uwe Völker, Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany ( lab head )

Submission Date

24/04/2017

Publication Date

16/05/2017

Tissue

saliva

Instrument

LTQ Orbitrap Velos

Software

Not available

Quantification

Not available

Experiment Type

Shotgun proteomics

Assay count

209

Publication

    Murr A, Pink C, Hammer E, Michalik S, Dhople VM, Holtfreter B, Völker U, Kocher T, Gesell Salazar M. Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education. J Proteome Res. 2017 May 8 PubMed: 28481548

Assay

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Showing 21 - 30 of 209 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
21 76160 11_batch2.dat-pride-filtered.xml 750 4019 1848 15517 0
22 76040 120_batch8.dat-pride-filtered.xml 817 4034 1904 16653 0
23 76161 122_batch7.dat-pride-filtered.xml 1022 5003 2413 17119 0
24 76041 123_batch7.dat-pride-filtered.xml 930 4239 2187 16127 0
25 76162 124_batch7.dat-pride-filtered.xml 982 4755 2301 16875 0
26 76042 125_batch7.dat-pride-filtered.xml 1153 5334 2704 16822 0
27 76163 126_batch7.dat-pride-filtered.xml 828 3961 1814 16551 0
28 76043 127_batch7.dat-pride-filtered.xml 773 3825 1775 16743 0
29 76164 128_batch7.dat-pride-filtered.xml 917 4533 2204 16203 0
30 76044 129_batch7.dat-pride-filtered.xml 1012 5044 2283 17404 0