Project PXD006367

PRIDE Assigned Tags:
Biological Dataset
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Summary

Title

Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education

Description

In a cross-sectional approach, we analyzed the influence of age, sex, body mass index (BMI), smoking, and education on salivary protein signatures in whole saliva samples of 187 individuals. Subjects were randomly selected from the population-based Study of Health in Pomerania (SHIP-Trend).

Sample Processing Protocol

- sample collection with Salivette (Sarstedt, Nümbrecht, Germany) - TCA precipitation of proteins - tryptic digestion - desalting of digests with ZipTip µC-18 - data dependent LC-MS/MS measurement on nano-UPLC/LTQ Orbitrap Velos

Data Processing Protocol

- data processing via Genedata Expressionist software package - identification via Mascot algorithm

Contact

Annette Murr, University Medicine Greifswald
Uwe Völker, Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany ( lab head )

Submission Date

24/04/2017

Publication Date

16/05/2017

Tissue

saliva

Instrument

LTQ Orbitrap Velos

Software

Not available

Quantification

Not available

Experiment Type

Shotgun proteomics

Assay count

209

Publication

    Murr A, Pink C, Hammer E, Michalik S, Dhople VM, Holtfreter B, Völker U, Kocher T, Gesell Salazar M. Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education. J Proteome Res. 2017 May 8 PubMed: 28481548

Assay

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# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 76140 100_batch9.dat-pride-filtered.xml 1346 6326 3140 16849 0
2 76141 101_batch9.dat-pride-filtered.xml 812 3949 1823 16458 0
3 76142 102_batch9.dat-pride-filtered.xml 1108 4778 2443 17013 0
4 76143 103_batch9.dat-pride-filtered.xml 1144 5392 2700 17306 0
5 76144 104_batch9.dat-pride-filtered.xml 976 4463 2215 16218 0
6 76145 105_batch9.dat-pride-filtered.xml 1059 4952 2546 17022 0
7 76146 106_batch9.dat-pride-filtered.xml 1105 4963 2600 17488 0
8 76147 107_batch9.dat-pride-filtered.xml 1100 4955 2519 16933 0
9 76148 108_batchR.dat-pride-filtered.xml 1077 5019 2545 16958 0
10 76149 109_batch9.dat-pride-filtered.xml 842 3652 1819 16496 0
11 76130 10_batch1.dat-pride-filtered.xml 666 3934 1604 15728 0
12 76131 110_batch8.dat-pride-filtered.xml 1018 4718 2401 16636 0
13 76132 111_batch8.dat-pride-filtered.xml 1111 5039 2609 17119 0
14 76133 112_batch8.dat-pride-filtered.xml 1015 4429 2330 17063 0
15 76134 113_batch8.dat-pride-filtered.xml 892 4081 2050 16332 0
16 76135 114_batch8.dat-pride-filtered.xml 1222 5526 2872 17224 0
17 76136 115_batch8.dat-pride-filtered.xml 1193 5460 2814 17030 0
18 76137 116_batch8.dat-pride-filtered.xml 833 3938 1899 16653 0
19 76138 117_batch8.dat-pride-filtered.xml 1053 5189 2537 17223 0
20 76139 118_batchR.dat-pride-filtered.xml 1160 5148 2777 17965 0
21 76160 11_batch2.dat-pride-filtered.xml 750 4019 1848 15517 0
22 76040 120_batch8.dat-pride-filtered.xml 817 4034 1904 16653 0
23 76161 122_batch7.dat-pride-filtered.xml 1022 5003 2413 17119 0
24 76041 123_batch7.dat-pride-filtered.xml 930 4239 2187 16127 0
25 76162 124_batch7.dat-pride-filtered.xml 982 4755 2301 16875 0
26 76042 125_batch7.dat-pride-filtered.xml 1153 5334 2704 16822 0
27 76163 126_batch7.dat-pride-filtered.xml 828 3961 1814 16551 0
28 76043 127_batch7.dat-pride-filtered.xml 773 3825 1775 16743 0
29 76164 128_batch7.dat-pride-filtered.xml 917 4533 2204 16203 0
30 76044 129_batch7.dat-pride-filtered.xml 1012 5044 2283 17404 0
31 76165 12_batch2.dat-pride-filtered.xml 902 5538 2171 16734 0
32 76045 130_batch7.dat-pride-filtered.xml 1104 5265 2655 17205 0
33 76166 131_batch7.dat-pride-filtered.xml 930 4210 2078 16494 0
34 76046 132_batch6.dat-pride-filtered.xml 1097 4936 2593 17254 0
35 76167 133_batch6.dat-pride-filtered.xml 786 3308 1727 15221 0
36 76047 134_batch6.dat-pride-filtered.xml 920 4234 2161 14851 0
37 76168 135_batch6.dat-pride-filtered.xml 368 1364 725 12394 0
38 76048 136_batch6.dat-pride-filtered.xml 850 3997 2063 16201 0
39 76169 137_batch6.dat-pride-filtered.xml 913 4201 2071 16415 0
40 76049 138_batch6.dat-pride-filtered.xml 1016 4437 2409 17182 0
41 76150 139_batch6.dat-pride-filtered.xml 909 4256 2154 16102 0
42 76151 13_batch2.dat-pride-filtered.xml 1019 5303 2494 16200 0
43 76152 140_batch6.dat-pride-filtered.xml 844 4191 1947 16647 0
44 76153 141_batch6.dat-pride-filtered.xml 807 3757 1844 16743 0
45 76154 142_batchR.dat-pride-filtered.xml 919 4032 2085 17401 0
46 76034 144_batch5.dat-pride-filtered.xml 1072 5193 2579 16641 0
47 76155 145_batch5.dat-pride-filtered.xml 1112 5527 2637 16653 0
48 76035 146_batch5.dat-pride-filtered.xml 900 4301 2040 15849 0
49 76156 147_batch5.dat-pride-filtered.xml 878 4086 2011 15982 0
50 76036 148_batch5.dat-pride-filtered.xml 994 5212 2394 15971 0