Project PXD006367

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Biological Dataset
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Summary

Title

Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education

Description

In a cross-sectional approach, we analyzed the influence of age, sex, body mass index (BMI), smoking, and education on salivary protein signatures in whole saliva samples of 187 individuals. Subjects were randomly selected from the population-based Study of Health in Pomerania (SHIP-Trend).

Sample Processing Protocol

- sample collection with Salivette (Sarstedt, Nümbrecht, Germany) - TCA precipitation of proteins - tryptic digestion - desalting of digests with ZipTip µC-18 - data dependent LC-MS/MS measurement on nano-UPLC/LTQ Orbitrap Velos

Data Processing Protocol

- data processing via Genedata Expressionist software package - identification via Mascot algorithm

Contact

Annette Murr, University Medicine Greifswald
Uwe Völker, Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany ( lab head )

Submission Date

24/04/2017

Publication Date

16/05/2017

Tissue

saliva

Instrument

LTQ Orbitrap Velos

Software

Not available

Quantification

Not available

Experiment Type

Shotgun proteomics

Assay count

209

Publication

    Murr A, Pink C, Hammer E, Michalik S, Dhople VM, Holtfreter B, Völker U, Kocher T, Gesell Salazar M. Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education. J Proteome Res. 2017 May 8 PubMed: 28481548

Assay

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# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 76140 100_batch9.dat-pride-filtered.xml 1346 6326 3140 16849 0
2 76141 101_batch9.dat-pride-filtered.xml 812 3949 1823 16458 0
3 76142 102_batch9.dat-pride-filtered.xml 1108 4778 2443 17013 0
4 76143 103_batch9.dat-pride-filtered.xml 1144 5392 2700 17306 0
5 76144 104_batch9.dat-pride-filtered.xml 976 4463 2215 16218 0
6 76145 105_batch9.dat-pride-filtered.xml 1059 4952 2546 17022 0
7 76146 106_batch9.dat-pride-filtered.xml 1105 4963 2600 17488 0
8 76147 107_batch9.dat-pride-filtered.xml 1100 4955 2519 16933 0
9 76148 108_batchR.dat-pride-filtered.xml 1077 5019 2545 16958 0
10 76149 109_batch9.dat-pride-filtered.xml 842 3652 1819 16496 0
11 76130 10_batch1.dat-pride-filtered.xml 666 3934 1604 15728 0
12 76131 110_batch8.dat-pride-filtered.xml 1018 4718 2401 16636 0
13 76132 111_batch8.dat-pride-filtered.xml 1111 5039 2609 17119 0
14 76133 112_batch8.dat-pride-filtered.xml 1015 4429 2330 17063 0
15 76134 113_batch8.dat-pride-filtered.xml 892 4081 2050 16332 0
16 76135 114_batch8.dat-pride-filtered.xml 1222 5526 2872 17224 0
17 76136 115_batch8.dat-pride-filtered.xml 1193 5460 2814 17030 0
18 76137 116_batch8.dat-pride-filtered.xml 833 3938 1899 16653 0
19 76138 117_batch8.dat-pride-filtered.xml 1053 5189 2537 17223 0
20 76139 118_batchR.dat-pride-filtered.xml 1160 5148 2777 17965 0
21 76160 11_batch2.dat-pride-filtered.xml 750 4019 1848 15517 0
22 76040 120_batch8.dat-pride-filtered.xml 817 4034 1904 16653 0
23 76161 122_batch7.dat-pride-filtered.xml 1022 5003 2413 17119 0
24 76041 123_batch7.dat-pride-filtered.xml 930 4239 2187 16127 0
25 76162 124_batch7.dat-pride-filtered.xml 982 4755 2301 16875 0
26 76042 125_batch7.dat-pride-filtered.xml 1153 5334 2704 16822 0
27 76163 126_batch7.dat-pride-filtered.xml 828 3961 1814 16551 0
28 76043 127_batch7.dat-pride-filtered.xml 773 3825 1775 16743 0
29 76164 128_batch7.dat-pride-filtered.xml 917 4533 2204 16203 0
30 76044 129_batch7.dat-pride-filtered.xml 1012 5044 2283 17404 0
31 76165 12_batch2.dat-pride-filtered.xml 902 5538 2171 16734 0
32 76045 130_batch7.dat-pride-filtered.xml 1104 5265 2655 17205 0
33 76166 131_batch7.dat-pride-filtered.xml 930 4210 2078 16494 0
34 76046 132_batch6.dat-pride-filtered.xml 1097 4936 2593 17254 0
35 76167 133_batch6.dat-pride-filtered.xml 786 3308 1727 15221 0
36 76047 134_batch6.dat-pride-filtered.xml 920 4234 2161 14851 0
37 76168 135_batch6.dat-pride-filtered.xml 368 1364 725 12394 0
38 76048 136_batch6.dat-pride-filtered.xml 850 3997 2063 16201 0
39 76169 137_batch6.dat-pride-filtered.xml 913 4201 2071 16415 0
40 76049 138_batch6.dat-pride-filtered.xml 1016 4437 2409 17182 0
41 76150 139_batch6.dat-pride-filtered.xml 909 4256 2154 16102 0
42 76151 13_batch2.dat-pride-filtered.xml 1019 5303 2494 16200 0
43 76152 140_batch6.dat-pride-filtered.xml 844 4191 1947 16647 0
44 76153 141_batch6.dat-pride-filtered.xml 807 3757 1844 16743 0
45 76154 142_batchR.dat-pride-filtered.xml 919 4032 2085 17401 0
46 76034 144_batch5.dat-pride-filtered.xml 1072 5193 2579 16641 0
47 76155 145_batch5.dat-pride-filtered.xml 1112 5527 2637 16653 0
48 76035 146_batch5.dat-pride-filtered.xml 900 4301 2040 15849 0
49 76156 147_batch5.dat-pride-filtered.xml 878 4086 2011 15982 0
50 76036 148_batch5.dat-pride-filtered.xml 994 5212 2394 15971 0
51 76157 149_batch5.dat-pride-filtered.xml 892 4671 2195 16384 0
52 76037 14_batch2.dat-pride-filtered.xml 691 3872 1678 15641 0
53 76158 150_batch5.dat-pride-filtered.xml 825 4137 2021 16238 0
54 76038 151_batch5.dat-pride-filtered.xml 1080 5340 2625 16338 0
55 76159 152_batch5.dat-pride-filtered.xml 1019 5067 2475 16547 0
56 76039 154_batchR.dat-pride-filtered.xml 1035 4868 2518 17570 0
57 76180 155_batch4.dat-pride-filtered.xml 1083 5178 2605 16707 0
58 76060 156_batch4.dat-pride-filtered.xml 1095 5507 2623 16775 0
59 76181 157_batch4.dat-pride-filtered.xml 1092 5274 2620 16349 0
60 76061 158_batch4.dat-pride-filtered.xml 1141 5854 2765 17135 0
61 76182 159_batch4.dat-pride-filtered.xml 976 5057 2343 16788 0
62 76062 15_batch2.dat-pride-filtered.xml 766 4157 1800 15046 0
63 76183 160_batch4.dat-pride-filtered.xml 678 3342 1604 15909 0
64 76063 161_batch4.dat-pride-filtered.xml 819 4437 1942 16318 0
65 76184 162_batch4.dat-pride-filtered.xml 1107 5681 2661 17413 0
66 76064 163_batch4.dat-pride-filtered.xml 792 4421 2007 16281 0
67 76185 164_batch3.dat-pride-filtered.xml 871 4442 2079 15505 0
68 76065 165_batch3.dat-pride-filtered.xml 1028 4958 2586 15485 0
69 76186 166_batch3.dat-pride-filtered.xml 908 5112 2237 16360 0
70 76066 167_batch3.dat-pride-filtered.xml 947 4647 2244 15858 0
71 76187 168_batch3.dat-pride-filtered.xml 810 4185 1982 16013 0
72 76067 169_batch3.dat-pride-filtered.xml 1027 5092 2484 16002 0
73 76188 16_batch2.dat-pride-filtered.xml 878 5361 2197 16150 0
74 76068 170_batch3.dat-pride-filtered.xml 892 4976 2200 16312 0
75 76189 171_batch3.dat-pride-filtered.xml 770 4023 1838 15998 0
76 76069 172_batchR.dat-pride-filtered.xml 1075 5253 2591 18995 0
77 76170 173_batch3.dat-pride-filtered.xml 995 5308 2384 16083 0
78 76050 174_batchR.dat-pride-filtered.xml 719 3556 1516 17141 0
79 76171 175_batch2.dat-pride-filtered.xml 1013 5013 2451 15558 0
80 76051 176_batch2.dat-pride-filtered.xml 723 4470 1713 15469 0
81 76172 177_batch2.dat-pride-filtered.xml 971 5234 2395 16322 0
82 76052 178_batchR.dat-pride-filtered.xml 700 3328 1492 16473 0
83 76173 179_batch2.dat-pride-filtered.xml 1004 5167 2416 16165 0
84 76053 17_batch2.dat-pride-filtered.xml 864 4567 2096 15344 0
85 76174 180_batch2.dat-pride-filtered.xml 563 3366 1393 14625 0
86 76054 181_batch2.dat-pride-filtered.xml 684 4258 1736 15358 0
87 76175 182_batch2.dat-pride-filtered.xml 878 5169 2113 16399 0
88 76055 183_batch2.dat-pride-filtered.xml 765 4442 1920 15714 0
89 76176 184_batch1.dat-pride-filtered.xml 816 5283 1992 16402 0
90 76056 186_batch1.dat-pride-filtered.xml 532 3697 1233 14260 0
91 76177 187_batch1.dat-pride-filtered.xml 698 4523 1736 14981 0
92 76057 188_batch1.dat-pride-filtered.xml 808 4664 2009 15900 0
93 76178 189_batch1.dat-pride-filtered.xml 632 4517 1587 15660 0
94 76058 18_batch2.dat-pride-filtered.xml 667 4016 1617 15123 0
95 76179 190_batch1.dat-pride-filtered.xml 636 3970 1642 15217 0
96 76059 191_batch1.dat-pride-filtered.xml 630 4397 1596 15449 0
97 76080 192_batch1.dat-pride-filtered.xml 810 4905 2015 15816 0
98 76081 193_batch1.dat-pride-filtered.xml 789 4862 1929 15619 0
99 76082 194_batch1.dat-pride-filtered.xml 767 5017 1910 15762 0
100 76083 19_batch2.dat-pride-filtered.xml 767 4866 1878 16095 0