Project PXD006367

PRIDE Assigned Tags:
Biological Dataset
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Summary

Title

Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education

Description

In a cross-sectional approach, we analyzed the influence of age, sex, body mass index (BMI), smoking, and education on salivary protein signatures in whole saliva samples of 187 individuals. Subjects were randomly selected from the population-based Study of Health in Pomerania (SHIP-Trend).

Sample Processing Protocol

- sample collection with Salivette (Sarstedt, Nümbrecht, Germany) - TCA precipitation of proteins - tryptic digestion - desalting of digests with ZipTip µC-18 - data dependent LC-MS/MS measurement on nano-UPLC/LTQ Orbitrap Velos

Data Processing Protocol

- data processing via Genedata Expressionist software package - identification via Mascot algorithm

Contact

Annette Murr, University Medicine Greifswald
Uwe Völker, Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany ( lab head )

Submission Date

24/04/2017

Publication Date

16/05/2017

Tissue

saliva

Instrument

LTQ Orbitrap Velos

Software

Not available

Quantification

Not available

Experiment Type

Shotgun proteomics

Assay count

209

Publication

    Murr A, Pink C, Hammer E, Michalik S, Dhople VM, Holtfreter B, Völker U, Kocher T, Gesell Salazar M. Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education. J Proteome Res. 2017 May 8 PubMed: 28481548

Assay

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Showing 1 - 10 of 209 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 76140 100_batch9.dat-pride-filtered.xml 1346 6326 3140 16849 0
2 76141 101_batch9.dat-pride-filtered.xml 812 3949 1823 16458 0
3 76142 102_batch9.dat-pride-filtered.xml 1108 4778 2443 17013 0
4 76143 103_batch9.dat-pride-filtered.xml 1144 5392 2700 17306 0
5 76144 104_batch9.dat-pride-filtered.xml 976 4463 2215 16218 0
6 76145 105_batch9.dat-pride-filtered.xml 1059 4952 2546 17022 0
7 76146 106_batch9.dat-pride-filtered.xml 1105 4963 2600 17488 0
8 76147 107_batch9.dat-pride-filtered.xml 1100 4955 2519 16933 0
9 76148 108_batchR.dat-pride-filtered.xml 1077 5019 2545 16958 0
10 76149 109_batch9.dat-pride-filtered.xml 842 3652 1819 16496 0