Project PXD005794

PRIDE Assigned Tags:
Biological Dataset
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Summary

Title

Proteogenomics of a Chernobyl's Microbacterium sp. A9 bacterial isolate

Description

This project is a proteomic comparison of Microbacterium sp. A9 exposed to 10 ┬ÁM nitrate uranyl versus control condition without uranyl. Three sampling time points (30 min, 4h and 24h)are analyzed.

Sample Processing Protocol

Protein samples were loaded on a SDS-PAGE, sliced into polyacrylamide bands, digested by trypsin, and the resulting peptides were analyzed by Q-Exactive HF tandem mass spectrometry.

Data Processing Protocol

MS/MS spectra were analyzed by MASCOT, the results were then parsed with IRMA, spectral counts were compared with PatternLab.

Contact

Jean ARMENGAUD, Li2D
Jean ARMENGAUD, CEA, DRF, IBiTec-S, SPI, Li2D, Laboratory "Innovative technologies for Detection and Diagnostics" ( lab head )

Submission Date

27/01/2017

Publication Date

30/11/2018

Tissue

Not available

Instrument

Q Exactive HF

Software

Not available

Modification

Carbamidomethyl
Oxidation

Quantification

Spectrum counting

Experiment Type

Shotgun proteomics

Assay count

48

Publication

Publication pending

Assay

Page 1 2 3 4 5
Page size 10 20
Showing 1 - 10 of 48 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 73902 F104903.mzid 1331 24946 8462 50602 0
2 73924 F104907.mzid 1226 23847 7630 46049 0
3 73901 F104909.mzid 1300 26475 8302 53057 0
4 73923 F104910.mzid 1215 24079 7429 45207 0
5 73900 F104912.mzid 1345 28572 8363 51535 0
6 73922 F104914.mzid 1405 33865 9413 56644 0
7 73921 F104916.mzid 1488 39214 10386 68402 0
8 73920 F104918.mzid 1320 31524 8619 49213 0
9 73919 F104921.mzid 1279 30762 8335 46244 0
10 73918 F104923.mzid 1413 36814 9626 61117 0