PRIDE Assigned Tags:Biomedical Dataset
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Proteomic Analysis of Hypoxic Human Lung Epithelial Cells
H441 cell proteome was sequenced via SILAC and LC-MS/MS method to identify hypoxia-responsive proteins
Sample Processing Protocol
Protein were isolated from normoxic or hypoxic cells followed by FASP digestion. Digested peptides were mixed in equal ratios.
Data Processing Protocol
MS/MS spectra were extracted, and raw files were searched against the most current Homo sapiens protein sequence database from UniprotKB using Maxquant (version 126.96.36.199) and Mascot Distiller (version 188.8.131.52) for quantification of labeled proteins.
Kathiriya JJ, Nakra N, Nixon J, Patel PS, Vaghasiya V, Alhassani A, Tian Z, Allen-Gipson D, Davé V. Galectin-1 inhibition attenuates profibrotic signaling in hypoxia-induced pulmonary fibrosis. Cell Death Discov. 2017 Apr 10;3:17010. eCollection 2017 PubMed: 28417017
|#||Accession||Title||Proteins||Peptides||Unique Peptides||Spectra||Identified Spectra||View in Reactome|
|1||69996||Rep 1 from SILAC Jaymin_ 20141108_H1(heavy)+N1(light)_1 and 14 more.mzid||16086||223749||25937||77545||0||
|2||69995||Rep 2 from SILAC Jaymin_ 20141108_H1(heavy)+N1(light)_1 and 14 more.mzid||15865||231379||24400||79016||0||
|3||69997||Rep 3 from SILAC Jaymin_ 20141108_H1(heavy)+N1(light)_1 and 14 more.mzid||15240||228667||23788||78496||0||