Project PXD004076

PRIDE Assigned Tags:
Biomedical Dataset

Summary

Title

Spatiotemporal proteomic profiling of human cerebral development

Description

We utilize a rapid shotgun LFQ MS method to profile four regions of developing human cerebrum. Regions encompass neural precursor rich ventricular zone, intermediate, subplate and cortex regions across human development from gestational week 16-36 and postnatal week 41 and adult brain tissue.

Sample Processing Protocol

Samples were processed using rapigest, an MS-compatible lysis reagent. Following sonication and incubation of samples at 95 degrees and 80 degrees for 1.5 hours samples were digested with trypsin and injected using microplate autosampler onto a C18 trap column using the EASY-nLC1000 system.

Data Processing Protocol

RAW Xcalibur MS spectra files were searched in MaxQuant (version 1.5.2.8) against the Human SpissProt database (January 2015 release).

Contact

Ugljesa Djuric, Mt. Sinai Hospital
Eleftherios Diamandis, Mount Sinai Hospital, Toronto, Ontario, Canada ( lab head )

Submission Date

31/03/2017

Publication Date

14/07/2017

Tissue

brain

Instrument

Q Exactive

Software

Not available

Quantification

Label free

Experiment Type

Shotgun proteomics

Publication

    Djuric U, Rodrigues DC, Batruch I, Ellis J, Shannon P, Diamandis P. Spatiotemporal proteomic profiling of human cerebral development. Mol Cell Proteomics. 2017 Jul 7. pii: mcp.M116.066274 PubMed: 28687556