Spatiotemporal proteomic profiling of human cerebral development
We utilize a rapid shotgun LFQ MS method to profile four regions of developing human cerebrum. Regions encompass neural precursor rich ventricular zone, intermediate, subplate and cortex regions across human development from gestational week 16-36 and postnatal week 41 and adult brain tissue.
Sample Processing Protocol
Samples were processed using rapigest, an MS-compatible lysis reagent. Following sonication and incubation of samples at 95 degrees and 80 degrees for 1.5 hours samples were digested with trypsin and injected using microplate autosampler onto a C18 trap column using the EASY-nLC1000 system.
Data Processing Protocol
RAW Xcalibur MS spectra files were searched in MaxQuant (version 184.108.40.206) against the Human SpissProt database (January 2015 release).
Djuric U, Rodrigues DC, Batruch I, Ellis J, Shannon P, Diamandis P. Spatiotemporal proteomic profiling of human cerebral development. Mol Cell Proteomics. 2017 Jul 7. pii: mcp.M116.066274 PubMed: 28687556