Project PXD004051

PRIDE Assigned Tags:
Biomedical Dataset
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Summary

Title

Insulin stimulated MCF7 breast cancer cells

Description

Proteome profile of MCF7 breast cancer cells stimulated with insulin and analyzed by nano-LC-ESI-MS/MS.

Sample Processing Protocol

MCF7 cells were cultured in EMEM with insulin (10 microgram/mL) and FBS (10 %), harvested at 70-80 % confluence, lysed with RIPA buffer supplemented with protease and phosphatase inhibitors, digested with trypsin (substrate:enzyme ratio 50:1 w/w), and analyzed by nano-LC-ESI-MS/MS interfaced to an LTQ Thermo Electron ion trap mass spectrometer.

Data Processing Protocol

Raw data were processed with Proteome Discoverer 1.4, using a Homo Sapiens protein database from UniProt (2015/20199 entries), minimum peptide length 6 amino acids, maximum peptide length 144, smallest mass 500 Da, largest mass 5000 Da, 2 missed cleavages allowed, precursor ion tolerance 2 Da, fragment ion tolerance 1 Da, FDR stringent 1%, FDR relaxed 3 %.

Contact

Iulia Lazar, Biological Sciences
Iulia M. Lazar, Virginia Tech ( lab head )

Submission Date

25/04/2016

Publication Date

30/11/2016

Cell Type

epithelial cell

Disease

breast cancer

Instrument

LTQ

Quantification

Not available

Experiment Type

Shotgun proteomics

Assay count

16

Publication

    Sarvaiya HA, Lazar IM. Insulin stimulated MCF7 breast cancer cells: Proteome dataset. Data Brief. 2016 Sep 22;9:579-584 PubMed: 27761513

Assay

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Showing 1 - 10 of 16 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 64121 Insulin stimulated MCF7 cells 515 897 646 4907 765
2 64122 Insulin stimulated MCF7 cells 478 820 627 4923 734
3 64120 Insulin stimulated MCF7 cells 416 821 515 5253 628
4 64130 Insulin stimulated MCF7 cells 374 699 435 4655 572
5 64125 Insulin stimulated MCF7 cells 379 663 444 4782 516
6 64131 Insulin stimulated MCF7 cells 264 430 331 6054 378
7 64126 Insulin stimulated MCF7 cells 94 146 84 4624 94
8 64123 Insulin stimulated MCF7 cells 158 262 161 5477 168
9 64124 Insulin stimulated MCF7 cells 472 975 612 5742 677
10 64129 Insulin stimulated MCF7 cells 570 1359 881 5939 1029