Project PXD003700

PRIDE Assigned Tags:
Biomedical Dataset

Summary

Title

Human Arginine Methylome

Description

Identification of arginine methylation sites in human HEK293 cells, yielding a total of >8.000 high-confident modification sites on >3.300 proteins

Sample Processing Protocol

Peptides are fractionated by HpH fractionation, arginine methylated peptides enriched by IP and subsequently analyzed by high-resolution mass spectrometry

Data Processing Protocol

All data are processed with the MaxQuant software suite

Contact

Michael L. Nielsen, NNF Center for Protein Research
Michael Lund Nielsen, Novo Nordisk Foundation Center for Protein Research ( lab head )

Submission Date

26/02/2016

Publication Date

30/09/2016

Instrument

Q Exactive

Software

Not available

Modification

monomethylated residue

Quantification

SILAC

Experiment Type

Shotgun proteomics

Publication

    Larsen SC, Sylvestersen KB, Mund A, Lyon D, Mullari M, Madsen MV, Daniel JA, Jensen LJ, Nielsen ML. Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells. Sci Signal. 2016 Aug 30;9(443):rs9 PubMed: 27577262