PRIDE Assigned Tags:Biomedical Dataset
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The proteomes of plasmodium falciparum mature male and female gametocytes
Gametocytes are obligate for the transmission of malaria parasite to mosquito. This study reports the first comprehensive proteomes of accurately separated mature male and female Plasmodium falciparum gametocytes using a transgenic line. the proteomes revealed significant differences that are consistent with the divergent functions of the two sexes. While the male proteome is enriched in proteins associated with flagella and genome replication, the female proteome is more abundant in proteins involved in metabolism, translation and organellar functions.
Sample Processing Protocol
The separated parasites were lysed in SDT-lysis buffer (4% SDS, 100 mM Tris/HCl pH 7.6, 0.1 M DTT) for complete dissolution and separated in a 10% Bis-Tris SDS-PAGE gel. Ten slices were excised in-gel digestion was done as described (Lasonder et al., 2002). The digests were analyzed by nano LC/MS/MS with a Waters NanoAcquity HPLC system interfaced to a Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific). Peptides were loaded on a trapping column and eluted over a 75 μm analytical column at 350 nL/min. MS and MS/MS were performed at 70,000 FWHM and 17,500 FWHM resolution, respectively. The fifteen most abundant ions were selected for MS/MS.
Data Processing Protocol
The LC/MS/MS data were searched against Uniprot P. falciparum protein database (v01/2014) with reversed entries in order to assess false discovery rate (FDR). Data were filtered at 0.7-1% protein and 0.1-0.2% peptide FDR. Search parameters included trypsin digestion (C-terminal K and R cleavage), full cleavage with 2 missed sites, fragment ion mass tolerance with tolerance of 0.02 Da, peptide mass tolerance of 10 ppm. Mascot DAT files were parsed into the Scaffold software for validation and filtering to create a non-redundant list per sample.
Miao J, Chen Z, Wang Z, Shrestha S, Li X, Li R, Cui L. Sex-Specific Biology of the Human Malaria Parasite Revealed from the Proteomes of Mature Male and Female Gametocytes. Mol Cell Proteomics. 2017 Jan 26. pii: mcp.M116.061804 PubMed: 28126901
|#||Accession||Title||Proteins||Peptides||Unique Peptides||Spectra||Identified Spectra||View in Reactome|
|1||61600||no assay title provided (mzIdentML)||426||3094||1579||1782||1782||
|2||61602||no assay title provided (mzIdentML)||407||2934||1958||2231||2231||
|3||61601||no assay title provided (mzIdentML)||482||3094||2240||2402||2402||
|4||61604||no assay title provided (mzIdentML)||513||3944||2780||3209||3209||
|5||61640||no assay title provided (mzIdentML)||528||4208||2563||3164||3164||
|6||61603||no assay title provided (mzIdentML)||540||5460||3095||4049||4049||
|7||61641||no assay title provided (mzIdentML)||674||4183||2779||3375||3375||
|8||61642||no assay title provided (mzIdentML)||640||3293||2194||2631||2631||
|9||61643||no assay title provided (mzIdentML)||553||2215||1429||1629||1629||
|10||61644||no assay title provided (mzIdentML)||384||1577||794||948||948||