Proteomics of Primary cells derived from Ovarian Cancer
We analyzed primary cells derived from patients with ovarian cancer. We compared OSE, FTE and EOC and we use quantitative propeomics followed by WB, IHC, TMA for validation of our findings. We also analyzed the phosphoproteome of OVCAR3.
Sample Processing Protocol
We started form 700 ug of proteins, enriched (or not) for phophorylated peptides by TiO2-based chromatography, and analyzed the peptide mixture on QExactive and QExavtive-HF from Thermofisher.
Data Processing Protocol
We used MaxQuant, Perseus, and the Bioconductor pachkage LIMNA in R for data analysis.
Francavilla C, Lupia M, Tsafou K, Villa A, Kowalczyk K, Rakownikow Jersie-Christensen R, Bertalot G, Confalonieri S, Brunak S, Jensen LJ, Cavallaro U, Olsen JV. Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep. 2017 Mar 28;18(13):3242-3256 PubMed: 28355574