Project PXD003356

PRIDE Assigned Tags:
Biomedical Dataset
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Summary

Title

Quantitative Profiling of Protein S-Glutathionylation during Nanoparticle-Induced Oxidative Stress

Description

Quantitative profiling of protein s-glutathionylation (SSG) reveals redox-dependent regulation of macrophage function during nanoparticle-induced oxidative stress

Sample Processing Protocol

Treated cells with 3 different nanoparticles (SiO2, Fe3O4, CoO) then enriched for SSG nanoparticles. Samples were labeled with 6-plex TMT then analyzed by LC-MS/MS

Data Processing Protocol

Data was searched with MSGF+ using PNNL's DMS Processing pipeline

Contact

Matthew Monroe, Pacific Northwest National Laboratory
Weijun Qian, Pacific Northwest National Laboratory, USA ( lab head )

Submission Date

16/12/2015

Publication Date

02/08/2016

Quantification

Not available

Experiment Type

Shotgun proteomics

Assay count

3

Publication

    Duan J, Kodali VK, Gaffrey MJ, Guo J, Chu RK, Camp DG, Smith RD, Thrall BD, Qian WJ. Quantitative Profiling of Protein S-Glutathionylation Reveals Redox-Dependent Regulation of Macrophage Function during Nanoparticle-Induced Oxidative Stress. ACS Nano. 2016 Jan 26;10(1):524-38 PubMed: 26700264

Assay

Showing 1 - 3 of 3 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 60598 no assay title provided (mzIdentML) 17291 48835 38698 46517 38925
2 60597 no assay title provided (mzIdentML) 17437 48943 38829 45818 38751
3 60596 no assay title provided (mzIdentML) 17097 47166 37357 44752 37627