Project PXD003109

PRIDE Assigned Tags:
Technical Dataset
Dataset Belongs to:
PRIME-XS Project

Summary

Title

UVPD applied to phosphoproteomics

Description

UV-photodissociation was incorporated into a phosphoproteomic workflow for the analysis of peptide PTMs.

Sample Processing Protocol

Stock solutions of phosphopeptides were diluted to a concentration 5 uM and directly infused into the mass spectrometer. HeLa digest was analyzed with LC-MS with UVPD

Data Processing Protocol

UVPD tandem mass spectra were analyzed using SEQUEST HT database algorithms and Proteome Discoverer 2.0.

Contact

Kyle Fort, Utrecht University
Kyle L. Fort, Utrecht University ( lab head )

Submission Date

27/10/2015

Publication Date

08/08/2017

Tissue

Not available

Instrument

Q Exactive

Software

Not available

Modification

phosphorylated residue

Quantification

Not available

Experiment Type

Shotgun proteomics

Publication

    Fort KL, Dyachenko A, Potel CM, Corradini E, Marino F, Barendregt A, Makarov AA, Scheltema RA, Heck AJ. Implementation of Ultraviolet Photodissociation on a Benchtop Q Exactive Mass Spectrometer and Its Application to Phosphoproteomics. Anal Chem. 2016 Feb 16;88(4):2303-10 PubMed: 26760441