UVPD applied to phosphoproteomics
UV-photodissociation was incorporated into a phosphoproteomic workflow for the analysis of peptide PTMs.
Sample Processing Protocol
Stock solutions of phosphopeptides were diluted to a concentration 5 uM and directly infused into the mass spectrometer. HeLa digest was analyzed with LC-MS with UVPD
Data Processing Protocol
UVPD tandem mass spectra were analyzed using SEQUEST HT database algorithms and Proteome Discoverer 2.0.
Fort KL, Dyachenko A, Potel CM, Corradini E, Marino F, Barendregt A, Makarov AA, Scheltema RA, Heck AJ. Implementation of Ultraviolet Photodissociation on a Benchtop Q Exactive Mass Spectrometer and Its Application to Phosphoproteomics. Anal Chem. 2016 Feb 16;88(4):2303-10 PubMed: 26760441