Project PXD003045

PRIDE Assigned Tags:
Technical Dataset Biomedical Dataset
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Project Peptide Table
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Summary

Title

SPE optimization for peptide sample preparation for urine peptidomics

Description

Development of a modified solid phase extraction (SPE) approach for improved peptide yield and analysis sensitivity with LC-MS based peptidomics of urine.

Sample Processing Protocol

Samples were digested with trypsin then analyzed by LC-MS/MS

Data Processing Protocol

Data was searched with MSGF+, SEQUEST, and MSAlign using PNNL's DMS Processing pipeline

Contact

Matthew Monroe, Pacific Northwest National Laboratory
Minnie M. Sarwal, UCSF School of Medicine, USA ( lab head )

Submission Date

13/10/2015

Publication Date

16/10/2015

Tissue

urine

Modification

Oxidation

Quantification

Not available

Experiment Type

Shotgun proteomics

Assay count

8

Publication

    Sigdel TK, Nicora CD, Hsieh SC, Dai H, Qian WJ, Camp DG 2nd, Sarwal MM; Optimization for peptide sample preparation for urine peptidomics., Clin Proteomics, 2014 Feb 25, 11, 1, 7, PubMed: 24568099

Assay

Showing 1 - 8 of 8 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 58518 no assay title provided (mzIdentML) 12361 22355 19594 19541 18060
2 58517 no assay title provided (mzIdentML) 12454 22287 19901 19979 18252
3 58519 no assay title provided (mzIdentML) 11997 21162 18744 18833 17206
4 58523 no assay title provided (mzIdentML) 13492 22622 20423 20767 18465
5 58522 no assay title provided (mzIdentML) 13593 22490 20299 20682 18613
6 58521 no assay title provided (mzIdentML) 13119 21658 19598 19846 17831
7 58520 no assay title provided (mzIdentML) 13067 21866 19748 19892 18083
8 58516 no assay title provided (mzIdentML) 12897 23816 21197 21086 19333