Project PXD002421

PRIDE Assigned Tags:
Biological Dataset
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Summary

Title

lncRNA pulldown

Description

lncRNA interactome study, MCF7 cells, proteomics Velos Pro

Sample Processing Protocol

BrdU pulldown, SDS-PAGE, in-gel digest, Velos Pro, spectral counting

Data Processing Protocol

MSConvert .raw to .mgf, MASCOT, in-house Python script for data assembly and spectral counting

Contact

Anton Poliakov, Pathology
Felix Feng, University of Michigan ( lab head )

Submission Date

24/06/2015

Publication Date

05/10/2016

Publication

    Niknafs YS, Han S, Ma T, Speers C, Zhang C, Wilder-Romans K, Iyer MK, Pitchiaya S, Malik R, Hosono Y, Prensner JR, Poliakov A, Singhal U, Xiao L, Kregel S, Siebenaler RF, Zhao SG, Uhl M, Gawronski A, Hayes DF, Pierce LJ, Cao X, Collins C, Backofen R, Sahinalp CS, Rae JM, Chinnaiyan AM, Feng FY. The lncRNA landscape of breast cancer reveals a role for DSCAM-AS1 in breast cancer progression. Nat Commun. 2016 Sep 26;7:12791 PubMed: 27666543

Assay

Showing 1 - 6 of 6 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 54141 lncRNA pulldown 314 5045 1303 45012 4171
2 54144 lncRNA pulldown 335 5146 1397 45012 4403
3 54145 lncRNA pulldown 463 5534 1763 44664 4634
4 54142 lncRNA pulldown 288 4457 1204 45312 3854
5 54143 lncRNA pulldown 326 5185 1386 44484 4511
6 54146 lncRNA pulldown 401 5106 1602 45024 4286