Project PXD002395

PRIDE Assigned Tags:
Biological Dataset

Summary

Title

11 human cell lines

Description

Re-submission of raw data from Geiger T et. al., Comparative proteomic analysis of eleven common cell lines reveals ubiquitous but varying expression of most proteins. Mol Cell Proteomics. 2012 Mar;11(3):M111.014050. doi: 10.1074/mcp.M111.014050. Epub 2012 Jan 25.

Sample Processing Protocol

cell lines used: A549, GAMG, HEK293, HeLa, HepG2, K562, MCF7, RKO, U2OS, LnCap and Jurkat LC-MS/MS Experiment Labeling Method Labelfree Enrichment no Protease Trypsin/P Separation SAX Instrument Orbitrap Velos with high-field analyzer

Data Processing Protocol

MaxQuant v1.4.1.2 was used to search the .Raw files. mqpar.xml is attached.

Contact

Chris Bielow, MDC Berlin
Stefan Kempa, MDC Berlin, Berlin, Germany ( lab head )

Submission Date

19/06/2015

Publication Date

21/07/2015

Corresponding dataset(s) in other omics resources

http://proteomecommons.org

Instrument

LTQ Orbitrap Velos

Software

Not available

Quantification

Not available

Experiment Type

Shotgun proteomics

Publication

    Geiger T, Wehner A, Schaab C, Cox J, Mann M; Comparative proteomic analysis of eleven common cell lines reveals ubiquitous but varying expression of most proteins., Mol Cell Proteomics, 2012 Mar, 11, 3, M111.014050, PubMed: 22278370