Project PXD001874

PRIDE Assigned Tags:
Biological Dataset

Summary

Title

Human Hepatocyte Proteome

Description

Here we report a comprehensive proteomic analysis of purified human hepatocytes and a human hepatoma cell line HepG2. The complete dataset comprises 9,400 proteins and provides quantitative depiction of the proteomes of hepatocytes and HepG2 cells at the protein titer and copy number dimensions.

Sample Processing Protocol

Filter aided sample preparation (FASP), Strom anion exchemge (SAX), LC-MS/MS on QExactive mass spectrometers

Data Processing Protocol

Data analysis was performed using MaxQuant version 1.2.6.20 using Anromeda search engine.

Contact

Mario Oroshi, Proteomics
Wisniewski Jacek R., Max Planck Institute for Biochemistry ( lab head )

Submission Date

06/03/2015

Publication Date

26/01/2016

Tissue

liver

Cell Type

hepatocyte

Instrument

Q Exactive

Software

Not available

Quantification

Not available

Experiment Type

Shotgun proteomics

Publication

    Wiśniewski JR, Vildhede A, Norén A, Artursson P. In-depth quantitative analysis and comparison of the human hepatocyte and hepatoma cell line HepG2 proteomes. J Proteomics. 2016 Jan 26. pii: S1874-3919(16)30019-7 PubMed: 26825538