Human Hepatocyte Proteome
Here we report a comprehensive proteomic analysis of purified human hepatocytes and a human hepatoma cell line HepG2. The complete dataset comprises 9,400 proteins and provides quantitative depiction of the proteomes of hepatocytes and HepG2 cells at the protein titer and copy number dimensions.
Sample Processing Protocol
Filter aided sample preparation (FASP), Strom anion exchemge (SAX), LC-MS/MS on QExactive mass spectrometers
Data Processing Protocol
Data analysis was performed using MaxQuant version 188.8.131.52 using Anromeda search engine.
Wiśniewski JR, Vildhede A, Norén A, Artursson P. In-depth quantitative analysis and comparison of the human hepatocyte and hepatoma cell line HepG2 proteomes. J Proteomics. 2016 Jan 26. pii: S1874-3919(16)30019-7 PubMed: 26825538