Project PXD001446

PRIDE Assigned Tags:
Biological Dataset

Summary

Title

T. pseudoethanolicus response to furfural stress - LC-MS/MS

Description

Identification of the furfural detoxifying machinery in T. pseudoethanolicus (Tps) by LC-MS/MS based proteomics for engineering cellulolytic microbes involved in bioethanol production.

Sample Processing Protocol

Tps cells were grown in biological triplicate, proteins extracted via SDS/TCA/Urea modified FASP, and analyzed by 24-hr MudPIT LC-MS. Orbitrap XL was operated in DDA using a high mass accuracy in MS1 scans and LTQ in MS2 scans.

Data Processing Protocol

Spectra assiged to peptide sequences via SEQUEST/DTASelect. Spectra counts were measured, assigned nSpC values, and imported into JMP genomics to assign significant changers across conditions via ANOVA. Semiquantitative analysis with replication.

Contact

Richard J. Giannone, Oak Ridge National Laboratory
Richard J. Giannone, Chemical Sciences Division, Oak Ridge National Laboratory ( lab head )

Submission Date

30/10/2014

Publication Date

19/10/2018

Tissue

Not available

Instrument

LTQ Orbitrap

Software

Not available

Quantification

Spectrum counting

Experiment Type

Shotgun proteomics

Publication

    Clarkson SM, Hamilton-Brehm SD, Giannone RJ, Engle NL, Tschaplinski TJ, Hettich RL, Elkins JG. A comparative multidimensional LC-MS proteomic analysis reveals mechanisms for furan aldehyde detoxification in Thermoanaerobacter pseudethanolicus 39E. Biotechnol Biofuels. 2014 7(1):165 PubMed: 25506391