Project PXD001152

PRIDE Assigned Tags:
Biological Dataset Biomedical Dataset

Summary

Title

PBC DIGE

Description

Analyzing serological differences between AMA positive and negative PBC using DIGE

Sample Processing Protocol

High-abundant serum protein were depleted by affinity column, then depleted sera were label with Cy of DIGE. After DIGE analysis, 28 proteins spots were found of statistical different dye intensities and which were identified by MS/MS.

Data Processing Protocol

MALDI-TOF/TOF-MS analyses were performed in a 4800 Proteomics Analyzer (Applied Biosystems). Database search was performed with MASCOT Daemon search engine 2.2 (Matrix Science) through the Global Protein Server version 3.6 (Applied Biosystems).

Contact

Chuiwen Deng, Peking Union Medical college hospital
Yongzhe Li, Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, China ( lab head )

Submission Date

16/07/2014

Publication Date

27/11/2014

Software

Not available

Quantification

Not available

Experiment Type

Shotgun proteomics

Publication

Publication pending