Project PXD000744

PRIDE Assigned Tags:
Biomedical Dataset
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Summary

Title

CNB Lipid Rafts reAnalysis.

Description

This dataset represents an example for a textbook: Genomics and Proteomics for Clinical Discovery and Development, http://www.springer.com/life+sciences/systems+biology+and+bioinformatics/book/978-94-017-9201-1 ReAnalysis of the work Proteomic analysis of apical microvillous membranes of syncytiotrophoblast cells reveals a high degree of similarity with lipid rafts. Experiment described at: Paradela et al. J Proteome Res. 2005 Nov-Dec;4(6):2435-41 PMID: 16335998. For protein annotations, revise, Medina-Aunon et at. Proteomics. 2010 Sep;10(18):3262-71 PMID: 20707001. Genomics and Proteomics for Clinical Discovery and Development Translational Bioinformatics Volume 6, 2014, pp 41-68

Sample Processing Protocol

No additional steps have been added. Functional and biological annotations have been retrieved using PIKE.

Data Processing Protocol

Peptide and protein identification: Mascot v2.4 - DB: Uniprot/SP v. 2014_01. PRIDE generation: PRIDE Converter2 v 2.0.20 ProteomeXchange submission: PX submission v.2.0 Protein annotations: PIKE annotations v.1.0

Contact

J. Alberto Medina-Aunon, Proteomics - Bioinformatics
J.P. Albar, CNB/CSIC - ProteoRed ( lab head )

Submission Date

06/02/2014

Publication Date

14/07/2014

Quantification

Not available

Experiment Type

Shotgun proteomics

Assay count

1

Publication

Publication pending

Assay

Showing 1 - 1 of 1 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 34697 microvillous membranes (MVM) isolated from human syncytiotrophoblast cells 89 828 144 533 533