Project PXD000656

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Biomedical Dataset
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Summary

Title

CSF proteome mapping: Human Plasma LC-MS/MS, Part 6

Description

40 ┬ÁL plasma from a pool of 20 neurologically healthy donors was separated into a bound and a depleted fraction using a MARS Hu-14 column. This experiment represents the bound fraction. Sample was in-solution trypsin digested and peptides were fractionated into 10 fractions by mixed-mode reversed phase anion exchange (MM(RP-AX) using a Promix MP column. Each fraction was analyzed separately by LC-MS/MS on an Orbitrap Velos Pro mass spectrometer. Resulting data was searched using SeachGui, summarized in PeptideShaker and exported to the CSF-PR.

Sample Processing Protocol

See details in reference(s) : 25038066

Data Processing Protocol

See details in reference(s) : 25038066

Contact

Astrid Guldbrandsen, PROBE

Submission Date

18/12/2013

Publication Date

04/08/2014

Publication

    Guldbrandsen A, Vethe H, Farag Y, Oveland E, Garberg H, Berle M, Myhr KM, Opsahl JA, Barsnes H, Berven FS. In-depth characterization of the cerebrospinal fluid proteome displayed through the CSF Proteome Resource (CSF-PR). Mol Cell Proteomics. 2014 Jul 18. pii: mcp.M114.038554 PubMed: 25038066

Assay

Showing 1 - 1 of 1 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 33323 Plasma mixed-mode Bound 23864 111189 51419 184982 100951