Proteome of the Human Corpus Callosum
Characterization of the human Anterior Temporal Lobe and Corpus Callosum: C-HPP
Sample Processing Protocol
Protein samples extracted from human brains in 7 M urea, 2 M thiourea,4% CHAPS, 2% ASB-14, and 70 mM DTT. Proteins were pre-fractionated by SDS-PAGE and submitted to in-gel digestion. Peptides were separated in a nano HPLC, coupled to an LTQ-Orbitrap XL mass spectrometer.
Data Processing Protocol
Mass spectra were extracted in the MS2 format using the PatternLab for Proteomics RawReader employing the NeXtProt database.
Martins-de-Souza D, Carvalho PC, Schmitt A, Junqueira M, Nogueira FC, Turck CW, Domont GB. Deciphering the human brain proteome: characterization of the anterior temporal lobe and corpus callosum as part of the Chromosome 15-centric Human Proteome Project. J Proteome Res. 2014 Jan 3;13(1):147-57 PubMed: 24274931