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Summary

Title

CHPP chr 1,8,20 proteome dataset using the HCC cell lines of Hep3B and MHCC97H, instrument is AB5600, part 3 of 3

Description

The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-solution protein digestion by Lys-C and trypsin at 37 degrees Celsius respectively. The tryptic peptides were cleaned by C18 Sep-Pak column (Waters UK Ltd, Manchester, UK). The mass spectrometer used in this dataset was Triple TOF 5600 (AB SCIEX, Concord, ON). The dataset was produced with two technical replicates and 24 fractions per repeat. The database searching procedure was achieved using Mascot v2.3. The database is Swiss-prot (release 2013 -6).

Sample Processing Protocol

See details in reference(s) : 24328083

Data Processing Protocol

See details in reference(s) : 24328083

Contact

Zhang Chengpu Zhang Chengpu, Beijing proteome research center

Submission Date

23/10/2013

Publication Date

19/12/2013

Tissue

liver

Quantification

Not available

Experiment Type

Bottom-up proteomics

Assay count

96

Publication

    Liu Y, Ying W, Ren Z, Gu W, Zhang Y, Yan G, Yang P, Liu Y, Yin X, Chang C, Jiang J, Fan F, Zhang C, Xu P, Wang Q, Wen B, Lin L, Wang T, Du C, Zhong J, Wang T, He QY, Qian X, Lou X, Zhang G, Zhong F. Chromosome-8-coded proteome of Chinese Chromosome Proteome Data set (CCPD) 2.0 with partial immunohistochemical verifications. J Proteome Res. 2014 Jan 3;13(1):126-36 PubMed: 24328083

Assay

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Showing 1 - 10 of 96 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 31835 97H 5600 1908 6310 2441 30181 5277
2 31836 97H 5600 1867 6266 2425 30747 5348
3 31837 97H 5600 2054 9369 2804 44906 7920
4 31838 97H 5600 2066 9135 2823 44883 7820
5 31839 97H 5600 2094 13355 2768 46564 10380
6 31840 97H 5600 2156 13870 2827 46280 10430
7 31841 97H 5600 2081 10049 2772 43947 8175
8 31842 97H 5600 2124 10007 2811 43687 8208
9 31843 97H 5600 2232 9009 3103 44615 7805
10 31844 97H 5600 2197 9120 3074 45225 8018