Download Project Files
Project Protein Table
Project Peptide Table
Visualize in PRIDE Inspector
Follow the next three steps to open your selected project or assay in PRIDE Inspector:

  • 1.

    Download, uncompress and open PRIDE Inspector
  • 2.

    Click in the magnifier on the left top corner, paste the project or assay that you would like to open in the search box, and hit search
  • 3.

    Click in the corresponding "Download" button to download the files and visualize them

Summary

Title

CHPP chr 1,8,20 proteome dataset using the HCC cell lines of Hep3B and MHCC97H, instrument is Q Exactive, part 2 of 3

Description

The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-solution protein digestion by Lys-C and trypsin at 37 degrees Celsius, respectively. The tryptic peptides were cleaned by C18 Sep-Pak column (Waters UK Ltd, Manchester, UK). The mass spectrometer used in this dataset was Q-Exactive. The dataset was produced with two technical replicates and 24 fractions per repeat. The database searching procedure was achieved using Mascot v2.3. The database is Swiss-prot (release 2013 -6).

Sample Processing Protocol

See details in reference(s) : 24328083

Data Processing Protocol

See details in reference(s) : 24328083

Contact

Zhang Chengpu Zhang Chengpu, Beijing proteome research center

Submission Date

21/10/2013

Publication Date

16/12/2013

Tissue

liver

Quantification

Not available

Experiment Type

Bottom-up proteomics

Assay count

96

Publication

    Liu Y, Ying W, Ren Z, Gu W, Zhang Y, Yan G, Yang P, Liu Y, Yin X, Chang C, Jiang J, Fan F, Zhang C, Xu P, Wang Q, Wen B, Lin L, Wang T, Du C, Zhong J, Wang T, He QY, Qian X, Lou X, Zhang G, Zhong F. Chromosome-8-coded proteome of Chinese Chromosome Proteome Data set (CCPD) 2.0 with partial immunohistochemical verifications. J Proteome Res. 2014 Jan 3;13(1):126-36 PubMed: 24328083

Assay

Page 1 2 3 4 5 ... 10
Page size 10 20 50
Showing 1 - 10 of 96 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 31657 fudan QE using 97H and Hep3B 1866 4664 2466 23583 4247
2 31658 fudan QE using 97H and Hep3B 1817 4465 2374 22496 4068
3 31659 fudan QE using 97H and Hep3B 1626 4190 2202 20202 3865
4 31660 fudan QE using 97H and Hep3B 1619 4340 2201 20593 3996
5 31661 fudan QE using 97H and Hep3B 1737 4575 2333 21435 4111
6 31662 fudan QE using 97H and Hep3B 1723 4642 2331 21370 4171
7 31663 fudan QE using 97H and Hep3B 1610 4121 2112 21967 3843
8 31664 fudan QE using 97H and Hep3B 1615 4306 2159 22628 3981
9 31665 fudan QE using 97H and Hep3B 1956 5230 2661 24323 4794
10 31666 fudan QE using 97H and Hep3B 1981 5324 2712 24816 4906