Project PXD000529

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Biomedical Dataset
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Summary

Title

CHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, instrument is Q Exactive, part 1 of 3

Description

The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-solution protein digestion by Lys-C and trypsin at 37 degrees Celsius, respectively. The tryptic peptides were cleaned by C18 Sep-Pak column (Waters UK Ltd, Manchester, UK).

The mass spectrometer used in this dataset was Q-Exactive. The dataset was produced with two technical replicates and 24 fractions per repeat. The database searching procedure was achieved using Mascot v2.3. The database is Swiss-prot (release 2013 -6).

Sample Processing Protocol

See details in reference(s) : 24328083

Data Processing Protocol

See details in reference(s) : 24328083

Contact

Zhang Chengpu Zhang Chengpu, Beijing proteome research center

Submission Date

18/10/2013

Publication Date

13/12/2013

Tissue

liver

Quantification

Not available

Experiment Type

Bottom-up proteomics

Assay count

96

Publication

    Liu Y, Ying W, Ren Z, Gu W, Zhang Y, Yan G, Yang P, Liu Y, Yin X, Chang C, Jiang J, Fan F, Zhang C, Xu P, Wang Q, Wen B, Lin L, Wang T, Du C, Zhong J, Wang T, He QY, Qian X, Lou X, Zhang G, Zhong F. Chromosome-8-coded proteome of Chinese Chromosome Proteome Data set (CCPD) 2.0 with partial immunohistochemical verifications. J Proteome Res. 2014 Jan 3;13(1):126-36 PubMed: 24328083

Assay

Page 1 2 3 4 5 ... 10
Page size 10 20 50
Showing 1 - 10 of 96 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 31515 qianlab QE using 97H and LM3 2917 6044 3894 18022 4781
2 31516 qianlab QE using 97H and LM3 2905 6002 3814 17785 4751
3 31517 qianlab QE using 97H and LM3 2637 5500 3592 17429 4595
4 31518 qianlab QE using 97H and LM3 2612 5308 3514 17266 4434
5 31519 qianlab QE using 97H and LM3 2680 5412 3557 17028 4389
6 31520 qianlab QE using 97H and LM3 2743 5527 3601 17324 4410
7 31521 qianlab QE using 97H and LM3 2606 5218 3412 16941 4164
8 31522 qianlab QE using 97H and LM3 2559 5088 3320 16224 4037
9 31523 qianlab QE using 97H and LM3 2669 5226 3368 16399 4059
10 31524 qianlab QE using 97H and LM3 2739 5443 3336 16935 4071