PRIDE > Archive > PXD000440 > 31010

Assay 31010

Download Assay Files
Assay Protein Table
Assay Peptide Table
Visualize in PRIDE Inspector
Follow the next three steps to open your selected project or assay in PRIDE Inspector:

  • 1.

    Download, uncompress and open PRIDE Inspector
  • 2.

    Click in the magnifier on the left top corner, paste the project or assay that you would like to open in the search box, and hit search
  • 3.

    Click in the corresponding "Download" button to download the files and visualize them

Statistics

Protein Count Peptide Count Unique Peptide Count Total Spectrum Count Identified Spectrum Count
168 417 195 14477 347

Summary

Title

iTRAQ8plex labelled F15R-Kit cells stimulated with control (n=2), IL-2 (n=3) or IL15 (n=3); Phosphotyrosine analysis

Short Label

iTRAQ8plex F15R-Kit cells IL-2 vs IL-15; Phosphotyrosine

Experimental Factor

Not available

Contact

Hannah Johnson, MIT
Abhinav Arneja, MIT
Forest M White, MIT

Instrument

Model Source Analyzer Detector
Orbitrap Elite CID/HCD Nanoelectrospray
LTQ Orbitrap Elite
orbitrap
ion trap

Additional Details

Name Value
Date of search 2012-11-03 17:22
Digital Object Identifier (DOI) 10.6019/PXD000440
Experiment description
Experiment description 3 biological replicates of F15R-Kit cells stimulated with 500pM IL-2 or IL-15 for 15 minutes, and two biological replicates of unstimulated cells (total 8 conditions) were iTRAQ8plex labeled, combined and analyzed together. Phosphotyrosine peptides were enriched for by immunoprecipitation followed by further enrichment by IMAC. Peptides were anaylzed on an Orbitrap Elite with CID and HCD scans for MS/MS peptide identification.
ms-ms search
Original MS data file format Mascot dat file
Project Qualitatively Different T Cell Phenotypic Responses to IL-2 versus IL-15 are Unified by Identical Dependences on Receptor Signal Strength and Duration
ProteomeXchange accession number PXD000440
submitter keyword iTRAQ8plex, Phosphotyrosine, Phosphorylation, Quantification, IL-2, IL-15,
XML generation software PRIDE Converter Toolsuite 2.0.19