Project PXD000384

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Biomedical Dataset
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Summary

Title

V5-tagged LIMD1 in HEK293 cells with or without JNK2 activation

Description

The experiment was conducted to identify JNK phosphorylation sites on human LIM domain containing protein-1 (LIMD1). V5-tagged LIMD1 was co-transfected or not with a constitutively active form of JNK2 and purified. The purified proteins were subjected to SDS-PAGE, protease K digestion and MS/MS analysis. The data were searched against Swissprot using Mascot server v2.3. The fixed modification was set to carbamidomethylation of C, and variable modifications were set to oxidation of M, phosphorylation of ST, phosphorylation of Y. Mass values were set as monoisotopic. Peptide mass tolerance was set to +/-10ppm, and MS/MS tolerance was set to +/-0.8Da.

Sample Processing Protocol

See details in reference(s) : 24023255

Data Processing Protocol

See details in reference(s) : 24023255

Contact

Gongping Sun, Waksman Institute of Microbiology

Submission Date

31/07/2013

Publication Date

09/07/2014

Cell Type

epithelial cell

Instrument

LTQ

Experiment Type

Bottom-up proteomics

Assay count

1

Publication

    Sun G, Irvine KD. Ajuba family proteins link JNK to Hippo signaling. Sci Signal. 2013 Sep 10;6(292):ra81 PubMed: 24023255

Assay

Showing 1 - 1 of 1 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 30469 LIMD1:V5 purified from HEK293 cells 80 356 199 9029 272