Project PXD000383

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Summary

Title

QUEST-MS for plasma biomarkers complementing the specificity of PSA test

Description

To explore plasma biomarkers complementing the specificity of PSA test, we developed a unique proteomic technology QUEST-MS (Quick Enrichment of Small Targets for Mass Spectrometry). The QUEST-MS method based on 96-well formatted sequential reversed phase chromatography allowing efficient enrichment of < 20 kDa proteins quickly and reproducibly. The LC/MS/MS data from 24 healthy controls, 19 benign prostate hypertrophy (BPH) patients, and 73 prostate cancer patients plasma were subjected to label-free quantification analysis on Expressionist proteome server. Data processing: Mascot Server 2.3.1 was used with the database SwissProt 2011_08 (531473 sequences,188463640 residues). Enzyme: semiTrypsin. Search parameters: Peptide Mass Tolerance : +- 3 ppm, Fragment Mass Tolerance:+-0.8 Da. Fixed modifications:Carbamidomethyl (C). Variable modifications:Oxidation (M),Deamidated (NQ). Max Missed Cleavages:2.

Sample Processing Protocol

See details in reference(s) : 23991666

Data Processing Protocol

See details in reference(s) : 23991666

Contact

Koji Ueda, Laboratory for Biomarker Development

Submission Date

30/07/2013

Publication Date

28/01/2014

Publication

    Ueda K, Tatsuguchi A, Saichi N, Toyama A, Tamura K, Furihata M, Takata R, Akamatsu S, Igarashi M, Nakayama M, Sato TA, Ogawa O, Fujioka T, Shuin T, Nakamura Y, Nakagawa H. Plasma low-molecular-weight proteome profiling identified neuropeptide-Y as a prostate cancer biomarker polypeptide. J Proteome Res. 2013 Oct 4;12(10):4497-506 PubMed: 23991666

Assay

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Showing 1 - 10 of 116 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 30353 QUEST-MS - Experiment 1 481 4940 1448 18989 3474
2 30354 QUEST-MS - Experiment 2 558 5286 1573 19577 3610
3 30355 QUEST-MS - Experiment 3 537 5348 1585 18789 3715
4 30356 QUEST-MS - Experiment 4 521 5084 1531 19387 3499
5 30357 QUEST-MS - Experiment 5 598 5732 1703 20344 3725
6 30358 QUEST-MS - Experiment 6 519 5208 1641 18848 3823
7 30359 QUEST-MS - Experiment 7 597 5394 1694 19495 3773
8 30360 QUEST-MS - Experiment 8 505 5180 1535 19532 3627
9 30361 QUEST-MS - Experiment 9 546 5355 1616 20614 3669
10 30362 QUEST-MS - Experiment 10 537 5509 1578 20458 3659