Project PXD000377

Summary

Title

Proteomic analysis of purified protein derivative of mycobacterium tuberculosis

Description

Proteomic analysis of Connaught Tuberculin 68 (PPD-CT68), a tuberculin preparation generated froM. tuberculosis, was carried out in this study. PPD-CT68 is the protein component of a commercially available tuberculin preparation, Tubersol, which is used for tuberculin skin testing. Using a high resolution LTQ-Orbitrap Velos mass spectrometer, we identified 265 different proteins. The identified proteins were compared with those identified from PPD M. bovis, PPD M. avium and PPD-S2 from previous mass spectrometry-based studies. In all, 142 proteins were fo PPDs (i.e. proteins in either PPD-CT68 or PPD-S2), 37 proteins were found to be shared with M. avium PPD and 80 were shared with M. bovis PPD. Alignment of PPD-CT68 proteins with proteins encoded by 24 lung infecting bacteria revealed a number of similar proteins (206 bacterial proteins shared epitopes with 47 PPD-CT68 proteins), which could potentially be involved in causing cross-reactivity

Sample Processing Protocol

See details in reference(s) : 23870090

Data Processing Protocol

See details in reference(s) : 23870090

Contact

Akhilesh Pandey, Johns Hopkins University

Submission Date

29/07/2013

Publication Date

23/01/2014

Tissue

Not available

Instrument

LTQ Orbitrap

Software

Not available

Quantification

Not available

Experiment Type

Bottom-up proteomics

Publication

    Prasad TS, Verma R, Kumar S, Nirujogi RS, Sathe GJ, Madugundu AK, Sharma J, Puttamallesh VN, Ganjiwale A, Myneedu VP, Chatterjee A, Pandey A, Harsha H, Narayana J. Proteomic analysis of purified protein derivative of Mycobacterium tuberculosis. Clin Proteomics. 2013 Jul 19;10(1):8 PubMed: 23870090