Project PXD000355

PRIDE Assigned Tags:
Biological Dataset
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Summary

Title

Proteomic identification of rainbow trout sperm proteins

Description

Proteomics represents a powerful tool for the analysis of fish spermatozoa, since these cells are transcriptionally inactive. The aim of the present study was to generate an inventory of the most prominent rainbow trout sperm proteins with the use of one-dimensional electrophoresis prefractionation combined with performance liquid chromatography electrospray ionization tandem mass spectrometry. This study provides the first in-depth analysis of the rainbow trout sperm proteome, with a total of 204 identified proteins. We found that rainbow trout spermatozoa are equipped with functionally diverse proteins related to energetic metabolism, signal transduction, protein turnover, transport, cytoskeleton, oxidative injures and stress and reproduction. The availability of a catalogue of rainbow trout sperm proteins provides a crucial tool for the understanding of fundamental molecular processes in fish spermatozoa for ongoing research in the development of novel markers of sperm quality and for the optimization of short- and long-term sperm preservation procedures.

Sample Processing Protocol

See details in reference(s) : 24687995

Data Processing Protocol

See details in reference(s) : 24687995

Contact

Joanna Nynca, Department of Gamete and Embryo Biology

Submission Date

23/10/2013

Publication Date

04/11/2013

Publication

    Nynca J, Arnold GJ, Fröhlich T, Otte K, Ciereszko A. Proteomic identification of rainbow trout sperm proteins. Proteomics. 2014 Mar 29 PubMed: 24687995

Assay

Showing 1 - 8 of 8 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 31933 Biological replicate 1 622 10665 714 508252 5051
2 31934 Biological replicate 2 995 9241 985 475987 4942
3 31935 Biological replicate 3 359 6596 592 552935 3228
4 31936 Biological replicate 4 1055 5607 938 527057 3157
5 31937 Biological replicate 1 2618 30104 993 508252 4072
6 31938 Biological replicate 2 3108 47248 1100 475987 4762
7 31939 Biological replicate 3 2258 27038 928 552935 3319
8 31940 Biological replicate 4 2842 23327 947 526102 2891