Project PXD000228

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Summary

Title

Stimulated Human Platelet Lysate: control, PAR1 and PAR4 stimulated

Description

LC-MS/MS analysis of Control, PAR1-stimulated and PAR4-stimulated platelet lysate digests. Control lysate digest peptides were labeled with "light", PAR1 lysate digest peptides were labeled with "intermediate" and PAR4 lysate digest peptides were labeled with "high" dimethyl labeled. Labeled digests were mixed in a 1:1:1 ratio, separated by SCX and the resulting (phosphopeptide-enriched) peptide fractions were analyzed by LC-MS/MS.

Sample Processing Protocol

See details in reference(s) : 24776597

Data Processing Protocol

See details in reference(s) : 24776597

Contact

O Bleijerveld, Faculty of Science

Submission Date

11/07/2013

Publication Date

11/05/2016

Publication

    van Holten TC, Bleijerveld OB, Wijten P, de Groot PG, Heck AJ, Barendrecht AD, Merkx TH, Scholten A, Roest M. Quantitative proteomics analysis reveals similar release profiles following specific PAR-1 or PAR-4 stimulation of platelets. Cardiovasc Res. 2014 Jul 1;103(1):140-6 PubMed: 24776597

Assay

Showing 1 - 8 of 8 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 30086 PAR1- and PAR4-induced platelet releasate proteome screen -Exp I (HCD fragmentation) 3716 89765 11082 206833 61083
2 30087 PAR1- and PAR4-induced platelet releasate proteome screen -Exp I (ETD Ion Trap fragmentation) 3395 51664 8333 102932 36051
3 30088 PAR1- and PAR4-induced platelet releasate proteome screen -Exp I (ETD fragmentation in FT) 709 3362 1108 14162 2675
4 30089 PAR1- and PAR4-induced platelet releasate proteome screen -Exp II (HCD fragmentation) 2796 53078 8086 191652 37412
5 30090 PAR1- and PAR4-induced platelet releasate proteome screen -Exp II (ETD fragmentation in FT) 1994 23904 3586 88678 16768
6 30091 PAR1- and PAR4-induced platelet releasate proteome screen -Exp III (HCD fragmentation) 2530 39917 6843 163533 28033
7 30092 PAR1- and PAR4-induced platelet releasate proteome screen -Exp III (ETD fragmentation in Ion Trap) 2349 25939 5077 73461 18218
8 30093 PAR1- and PAR4-induced platelet releasate proteome screen -Exp III (ETD fragmentation in FT) 211 549 241 9267 461