Project PXD000018

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Summary

Title

Proteomic analysis of integrin adhesion complex-mediated mechanotransduction

Description

Integrin adhesion complexes were isolated from mouse fibroblasts treated with or without blebbistatin. Adhesion complexes were resolved by SDS-PAGE, digested with trpysin and analysed by LC-MS/MS using an LTQ Velos mass spectrometer

Sample Processing Protocol

Not available

Data Processing Protocol

Not available

Contact

Adam Byron, Faculty of Life Sciences

Submission Date

23/08/2012

Publication Date

19/10/2015

Tissue

Not available

Cell Type

fibroblast

Quantification

Not available

Experiment Type

Bottom-up proteomics

Assay count

8

Publication

Publication pending

Assay

Showing 1 - 8 of 8 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 27162 Fibronectin sample, blebbistatin-treated, biological replicate 1 4067 135234 6706 434534 36329
2 27163 Fibronectin sample, blebbistatin-treated, biological replicate 2 5957 158754 9646 445894 39565
3 27164 Fibronectin sample, control, biological replicate 1 5009 201893 8718 454848 45711
4 27165 Fibronectin sample, control, replicate 2 5817 177955 9849 465540 43042
5 27166 Transferrin sample, blebbistatin-treated, biological replicate 1 3252 74601 4327 392963 20394
6 27167 Transferrin sample, blebbistatin-treated, biological replicate 2 1906 24270 2360 389164 7294
7 27168 Transferrin sample, control, biological replicate 1 4292 109686 6097 412923 27721
8 27169 Transferrin sample, control, biological replicate 2 4040 76286 5067 407345 20963