PDBsum entry 9omc

Hydrolase

PDB id: 9omc

Contents

Protein chains

269 a.a.

Ligands

BKP ×2

EPE

Metals

_MN ×4

_MG ×2

Waters ×367

PDB id:

9omc

Name:

Hydrolase

Title:

Crystal structure of e. Coli apah in complex with gp4g

Structure:

Bis(5'-nucleosyl)-tetraphosphatase [symmetrical]. Chain: a, b. Synonym: ap4a hydrolase,diadenosine 5',5'''-p1,p4-tetraphosphate pyrophosphohydrolase,diadenosine tetraphosphatase. Engineered: yes

Source:

Escherichia coli k-12. Organism_taxid: 83333. Gene: apah, b0049, jw0048. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

2.05Å R-factor: 0.182 R-free: 0.204

Authors:

A.Nuthanakanti,A.Serganov

Key ref:

A.Nuthanakanti et al. Apah decaps np 4 n-Capped rnas in two alternative orientations.. Nat.Chem.Biol., . PubMed id: 40789943 DOI: 10.1038/S41589-025-01991-4

Date:

13-May-25 Release date: 03-Sep-25

Protein chains

P05637  (APAH_ECOLI) -  Bis(5'-nucleosyl)-tetraphosphatase [symmetrical] from Escherichia coli (strain K12)

Seq: 280 a.a.

Struc: 269 a.a.

Enzyme reactions

• Enzyme class: E.C.3.6.1.41 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical).
• Reaction: P₁,P₄-bis(5'-adenosyl) tetraphosphate + H2O = 2 ADP + 2 H⁺

P(1),P(4)-bis(5'-adenosyl) tetraphosphate + H2O = 2 × ADP (Bound ligand (Het Group name = BKP) matches with 75.00% similarity) + 2 × H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site