PDBsum entry 9jts

DNA binding protein/DNA

PDB id: 9jts

Contents

Protein chains

618 a.a.

344 a.a.

DNA/RNA

Metals

_ZN ×2

_CA ×2

PDB id:

9jts

Name:

DNA binding protein/DNA

Title:

Cryoem structure of mouse rag sec-1dna (12rss side)

Structure:

V(d)j recombination-activating protein 1. Chain: a, c. Synonym: rag-1. Engineered: yes. V(d)j recombination-activating protein 2. Chain: b, d. Synonym: rag-2. Engineered: yes. DNA (5'-d(p Tp Gp Gp Ap Tp Cp Tp Gp Gp Cp Cp Tp G)-3').

Source:

Mus musculus. House mouse. Organism_taxid: 10090. Gene: rag1. Expressed in: homo sapiens. Expression_system_taxid: 9606. Gene: rag2, rag-2. Synthetic: yes. Escherichia coli.

Authors:

X.Chen,L.Yao,W.Yang,M.Gellert

Key ref:

X.Chen et al. Cryoem structure of mouse rag sec-12DNA. To be published, .

Date:

07-Oct-24 Release date: 23-Jul-25

Protein chains

P15919  (RAG1_MOUSE) -  V(D)J recombination-activating protein 1 from Mus musculus

Seq: 1040 a.a.

Struc: 618 a.a.

Protein chains

P21784  (RAG2_MOUSE) -  V(D)J recombination-activating protein 2 from Mus musculus

Seq: 527 a.a.

Struc: 344 a.a.

DNA/RNA chains

T-G-G-A-T-C-T-G-G-C-C-T-G 13 bases

G-G-T-T-T-T-T-G-T-C-T-G-G-C-T-T-C-A-C-A-C-T-T-G-A-T-T-T-G-C-A-T-C-A-C-T-G-T-G 39 bases

C-A-C-A-G-T-G-A-T-A-C-A-G-C-C-C-T-T-A-A-C-A-A-A-A-A-C-C-C-G 30 bases

C-A-C-A-G-T-G-A-T-G-C-A-A-A-T-C-A-A-G-T-G-T-G-A-A-G-C-C-A-G-A-C-A-A-A-A-A-C-C 39 bases

C-A-G-G-C-C-A-G-A-T-C-C-A 13 bases

C-G-G-G-T-T-T-T-T-G-T-T-A-A-G-G-G-C-T-G-T-A-T-C-A-C-T-G-T-G 30 bases

Enzyme reactions

• Enzyme class: Chains A, C: E.C.3.1.-.-,2.3.2.27