PDBsum entry 9ibh

RNA binding protein

PDB id: 9ibh

Contents

Protein chains

545 a.a.

55 a.a.

Waters ×47

PDB id:

9ibh

Name:

RNA binding protein

Title:

Salmonella typhimurium polynucleotide phosphorylase in complex with recognition site of rnase e

Structure:

Polyribonucleotide nucleotidyltransferase. Chain: a, b, c. Engineered: yes. Other_details: core of the enzyme without kh and s1 domains. Ribonuclease e. Chain: d. Synonym: rnase e. Engineered: yes. Other_details: c-terminal segment of ribonuclease e

Source:

Salmonella enterica subsp. Enterica serovar typhimurium. Organism_taxid: 90371. Gene: pnp, stm3282. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: rne, cai81_13325. Expression_system_taxid: 469008

Authors:

G.Paris,B.F.Luisi

Key ref:

G.Paris and b.f.luisi Salmonella typhimurium polynucleotide phosphorylase i complex with recognition site of rnase e. To be published, .

Date:

12-Feb-25 Release date: 30-Jul-25

Protein chains

Q8ZLT3  (PNP_SALTY) -  Polyribonucleotide nucleotidyltransferase from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Seq: 711 a.a.

Struc: 545 a.a.

Protein chain

A0A8E6JFQ0  () -  Ribonuclease E from Salmonella enterica subsp. enterica serovar Typhimurium str. CFSAN000648

Seq: 1067 a.a.

Struc: 55 a.a.

Enzyme reactions

• Enzyme class 2: Chains A, B, C: E.C.2.7.7.8 - polyribonucleotide nucleotidyltransferase.
• Reaction: RNA(n+1) + phosphate = RNA(n) + a ribonucleoside 5'-diphosphate
• Enzyme class 3: Chain D: E.C.3.1.26.12 - ribonuclease E.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site